RAW 264.7 Cell Line murine

91062702, mouse blood, Macrophage

• 동의어(들): RAW264 7 Cells, RAW264.7 Cells
• UNSPSC 코드: 41106514

속성

• product name: RAW 264.7 Cell Line murine, Macrophage from blood, 91062702
• 생물학적 소스: mouse blood
• 성장 모드: Semi-adherent
• 핵형: Not specified
• 형태학: Macrophage
• 제품: Lysozyme
• 수용체: Immunoglobulin, complement
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse monocyte macrophage

세포주 설명

Established from an ascites of a tumour induced in a male mouse by intraperitoneal injection of Abselon Leukaemia Virus (A-MuLV). Cells will pinocytose neutral red and phagocytose zymosan. Cells capable of antibody dependent lysis of sheep erythrocytes and tumour targets. Growth inhibited by LPS.

애플리케이션

Metabolic studies

RAW 264.7 cell line has been used to study the anti-inflammatory effects of pristimerin. It has also been used to test the internalization and intracellular survival of Listeria monocytogenes.

배양 배지

DMEM + 2mM Glutamine + 10% FBS / FCS (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:2 to 1:8 i.e. seeding at 2-4x10,000 cells/cm²; 5% CO₂; 37°C. Remove the cells mechanically. Cells are semi-adherent, i.e. some cells grow in suspension, some loosely attach to the surface and others flattened out and attached to the flask. Cells should not be allowed to overgrow and become confluent as this can lead to loss of the flattened adherent cell characteristic.

기타 정보

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