RAW 264.7 Cell Line murine

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA. 91062702, mouse blood, Macrophage

• 동의어(들): RAW264 7 Cells, RAW264.7 Cells
• UNSPSC 코드: 41106514

속성

• 생물학적 소스: mouse blood
• 포장: tube of 5 μg 91062702-DNA-5UG; vial of cells 91062702-1VL
• 성장 모드: Semi-adherent
• 핵형: Not specified
• 형태학: Macrophage
• 제품: Lysozyme
• 수용체: Immunoglobulin, complement
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse monocyte macrophage

세포주 설명

Established from an ascites of a tumour induced in a male mouse by intraperitoneal injection of Abselon Leukaemia Virus (A-MuLV). Cells will pinocytose neutral red and phagocytose zymosan. Cells capable of antibody dependent lysis of sheep erythrocytes and tumour targets. Growth inhibited by LPS.

애플리케이션

Metabolic studies

RAW 264.7 cell line has been used to study the anti-inflammatory effects of pristimerin. It has also been used to test the internalization and intracellular survival of Listeria monocytogenes.

배양 배지

DMEM + 2mM Glutamine + 10% FBS / FCS (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:2 to 1:8 i.e. seeding at 2-4x10,000 cells/cm²; 5% CO₂; 37°C. Remove the cells mechanically. Cells are semi-adherent, i.e. some cells grow in suspension, some loosely attach to the surface and others flattened out and attached to the flask. Cells should not be allowed to overgrow and become confluent as this can lead to loss of the flattened adherent cell characteristic.

기타 정보

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.