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Key Documents

CMC0024

Sigma-Aldrich

CONTROLLER BL21(DE3) Chemically Competent Cells

Escherichia coli, rod shaped

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About This Item

UNSPSC 코드:
12352200
NACRES:
NA.85

product name

CONTROLLER BL21(DE3) Chemically Competent Cells, for controlled protein expression

생물학적 소스

Escherichia coli

Grade

for molecular biology

성장 모드

adherent or suspension

형태학

rod shaped

기술

microbiological culture: suitable

세포 형질전환

competent cell type: chemically competent
transformation efficiency: >1 x 107 cfu/μg

배송 상태

dry ice

저장 온도

−70°C

일반 설명

The CONTROLLER BL21(DE3) Chemically Competent Cells are an E. coli strain optimized for high efficiency transformation.
They are ideal for cloning and propagation of plasmid clones. They give high yield and high quality plasmid DNA.
Genotype

F- ompT hsdSB (rB- mB-) gal dcm (DE3) Mini-F lacIq1(GentR)

특징 및 장점

  • Reduce leaky expression: The LacI repressor protein binds to the lacO operator to block transcription of downstream genes. In the absence of an induction agent such as lactose sugar or IPTG, target protein expression from the adjacent promoter will be greatly reduced.
  • High protein yields: Both 10G and BL21(DE3)-based strains are noted for their ability to express high levels of protein. For expression from a T7 promoter please select the HI-Control BL21(DE3) strain. This strain also is deficient in proteases, yielding more stable protein preparations. Most other promoters can be used in either HI-Control strain.
  • Terrific efficiency: The simple, reliable heat-shock procedure for chemical transformation gives excellent results from small amounts of plasmid DNA.

성분

  • CONTROLLER BL21(DE3) chemically competent cells
  • pUC 19 transformation control DNA
  • recovery medium for expression


Storage Class Code

10 - Combustible liquids


시험 성적서(COA)

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M S Nadeem et al.
Brazilian journal of biology = Revista brasleira de biologia, 82, e244735-e244735 (2021-06-03)
L-Asparaginase catalysing the breakdown of L-Asparagine to L-Aspartate and ammonia is an enzyme of therapeutic importance in the treatment of cancer, especially the lymphomas and leukaemia. The present study describes the recombinant production, properties and anticancer potential of enzyme from

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