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Merck
모든 사진(2)

주요 문서

L2897

Sigma-Aldrich

LB Broth with agar (Lennox)

Highly-referenced microbial growth powder medium with Agar, low salt, suitable for salt-sensitive E. coli culture.

동의어(들):

Lennox broth, Luria Bertani Agar

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About This Item

UNSPSC 코드:
41106200
NACRES:
NA.85

Grade

for molecular biology

Quality Level

무균

non-sterile

양식

powder

구성

Agar, 15 g/L
NaCl, 5 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L

기술

microbiological culture: suitable

응용 분야

food and beverages
microbiology

저장 온도

room temp

적합성

nonselective for Escherichia coli
nonselective for coliforms

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일반 설명

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids and carbohydrates in a low-salt formulation.

애플리케이션

LB Broth with agar (Lennox) has been used for:

  • the transformation of competent E. coli cells
  • to cultivate Ecc-15 bacteria
  • embedding scaffold containing tissue derived from induced pluripotent stem cells (iPSCs)
  • transmission electron microscopy (TEM) and immunohistochemistry (IHC)
It is suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.

특징 및 장점

Lennox LB powder with agar provides:
  • A budget-friendly alternative to pre-poured plates
  • Easy scale-up using larger package sizes
  • Standard formulation

제조 메모

1. Suspend 35 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121°C.
To prepare Lennox L Agar: Add 1 g glucose and proceed with preparation instructions as above.To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml sterile 1 M magnesium sulfate after autoclaving.

법적 정보

Zeocin is a registered trademark of Cayla Sarl

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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프로토콜

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