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Merck
모든 사진(1)

주요 문서

L2542

Sigma-Aldrich

LB Broth (Miller)

Liquid microbial growth medium

동의어(들):

Luria Broth Miller, Luria-Bertani broth, Miller

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About This Item

UNSPSC 코드:
41106200
NACRES:
NA.85

Grade

for molecular biology

Quality Level

무균

sterile; 0.2 μm filtered

양식

liquid

기술

microbiological culture: suitable

pH

6.5-7.2

응용 분야

microbiology

저장 온도

room temp

적합성

nonselective for Escherichia coli
nonselective for coliforms

일반 설명

Miller’s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. It enables fast growth, improves growth yields for various bacterial species, and can retain the hydrophilic behavior of samples for longer periods.

애플리케이션

LBBroth (Miller) is suitable for:

  • non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.
  • selective cultivation when appropriate antibiotics are added.
  • the cultivation of E-coli cells for anti-bacterial assays.

특징 및 장점

Miller′s LB liquid provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package size
  • Standard formulation

성분

10g/L Tryptone
10 g/L NaCl
5 g/L Yeast Extract

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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시험 성적서(COA)

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Celine Abueva et al.
International journal of biological macromolecules, 182, 1713-1723 (2021-05-30)
The importance of developing more potent antimicrobials and robust infection prevention practices has been highlighted recently with the increase in reports of emerging bacterial resistance mechanisms and the development of antibiotic-resistant microbes. In this study, a quaternary ammonium chitosan derivative
Thomas P Burg et al.
Nature, 446(7139), 1066-1069 (2007-04-27)
Nanomechanical resonators enable the measurement of mass with extraordinary sensitivity. Previously, samples as light as 7 zeptograms (1 zg = 10(-21) g) have been weighed in vacuum, and proton-level resolution seems to be within reach. Resolving small mass changes requires
Development of mesoporous borosilicate bioactive glass nanoparticles containing Mg2+ and Zn2+: biocompatibility, bioactivity and antibacterial activity
Damian-Buda A I
Journal of Non-Crystalline Solids, 594, 121819-121819 (2022)
Andrada-Ioana Damian-Buda et al.
Journal of functional biomaterials, 13(4) (2022-10-25)
Mesoporous bioactive glass nanoparticles (MBGNs) are widely recognized for their ability to bond to hard tissue, while the ions released from the BG structure enhance specific cellular pathways. In this study, the SiO2-P2O5-CaO-MgO-ZnO system was used to successfully synthesize MBGNs
Long-term retention of hydrophilic behavior of plasma treated polydimethylsiloxane (PDMS) surfaces stored under water and Luria-Bertani broth
Zhao LH, et al.
Sensors and actuators A, Physical, 181.0, 33-42 (2012)

문서

유전 공학 및 복제의 발전은 연구 목적을 위한 이형 Protein Expression Systems 및 분리에 수많은 가능성을 열어주었습니다. 상당한 수준의 기술 발전으로 재조합형 단백질의 대규모 발현과 분리가 가능하게 되었습니다.

Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.

프로토콜

General protocols for growth of competent cells in microbial medium.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.