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Merck
모든 사진(2)

문서

L7658

Sigma-Aldrich

LB Broth (Lennox)

EZMix powder microbial growth medium

동의어(들):

Lennox broth

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About This Item

UNSPSC 코드:
41106200
NACRES:
NA.85

Grade

for molecular biology

Quality Level

설명

quick-dissolve, dust-free formulation

무균

non-sterile

형태

powder

기술

microbiological culture: suitable

pH

6.8-7.2(2.5% solution)

응용 분야

agriculture
food and beverages
microbiology

저장 온도

room temp

적합성

nonselective for Escherichia coli
nonselective for coliforms

일반 설명

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation.

애플리케이션

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.

특징 및 장점

Lennox LB EZMix powder provides:
  • Granulated, dust-free format for safer handling and faster mixing
  • Convenient small package to eliminate weighing
  • Easy scale-up using larger package sizes
  • Standard formulation

성분

*Note some of the product sizes available are given in liquid measure. This is a powder format product and those sizes reflect the final reconstituted volume.

10g/L Tryptone
5 g/L Yeast Extract
5 g/L NaCl
0.6 g/L inert binder (EZMix formulation only)

제조 메모

1. Suspend 20.6 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121 °C.
To prepare Lennox L Broth: Add 1 g glucose and proceed with preparation instructions as above.
To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml of sterile 1M magnesium sulfate after autoclaving.

재구성

Stir to suspend 20.6g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

법적 정보

Zeocin is a registered trademark of Cayla Sarl

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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Nature protocols, 5(4), 773-790 (2010-04-03)
Protein microarrays provide an efficient way to identify and quantify protein-protein interactions in high throughput. One drawback of this technique is that proteins show a broad range of physicochemical properties and are often difficult to produce recombinantly. To circumvent these
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프로토콜

General protocols for growth of competent cells in microbial medium.

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