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Merck
모든 사진(1)

문서

L3272

Sigma-Aldrich

LB Broth with agar (Luria low salt)

Powder microbial growth medium

동의어(들):

Luria Agar Base (Miller’s Modification)

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About This Item

UNSPSC 코드:
41106200
NACRES:
NA.85

Grade

for molecular biology

Quality Level

무균

non-sterile

형태

powder

구성

Agar, 15 g/L
NaCl, 0.5 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L

기술

microbiological culture: suitable

pH

6.8-7.2(3.05% solution)

응용 분야

food and beverages
microbiology

저장 온도

room temp

적합성

nonselective for Escherichia coli
nonselective for coliforms

일반 설명

Luria′s low salt LB is a microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. The addition of agar provides a solid medium for microbial growth.

애플리케이션

LB Broth with agar (Luria low salt) has been used:
  • for culturing E. coli DH10B cells.
  • in the isolation of diazotrophic bacteria.
  • for transformation of competent E. coli strain Stbl3 for cloning.
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.

특징 및 장점

Luria′s low salt LB powder with agar provides:
  • Easy scale-up using larger package sizes
  • A budget-friendly alternative to pre-poured plates
  • Standard formulation

제조 메모

1. Suspend 30.5 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve.
3. Autoclave for 15 minutes at 121°C.
4. Cool to 50°C prior to dispensing into petri dishes.

법적 정보

Zeocin is a registered trademark of Cayla Sarl

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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프로토콜

General protocols for growth of competent cells in microbial medium.

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