L3397

LB Broth (Luria low salt)

Powder microbial growth medium

• 동의어(들): Luria Broth (Miller’s Modification), Luria-Bertani medium (LB)
• UNSPSC 코드: 41106200
• NACRES: NA.85

속성

• Grade: for molecular biology
• Quality Level: 200
• 무균: non-sterile
• 형태: powder
• 구성: NaCl, 0.5 g/L ; Tryptone, 10 g/L ; Yeast Extract, 5 g/L
• 기술: microbiological culture: suitable
• pH: 6.8-7.2(1.55% solution)
• 적합성: suitable for molecular biology; nonselective for Escherichia coli; nonselective for coliforms
• 응용 분야: food and beverages; microbiology
• 저장 온도: room temp

설명

일반 설명

Luria Broth is a bacterial culture medium used for the cultivation of members of the Enterobacteriaceae especially E.coli, as well as for coliphage plaque assays. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a very low-salt formulation.

애플리케이션

LB Broth (Luria low salt) has been used as a liquid broth medium in the broth microdilution technique.

특징 및 장점

Luria′s low salt LB powder provides:

• Standard formulation
• A budget-friendly alternative to liquid
• Easy scale-up using larger package sizes

제조 메모

1. Suspend 15.5 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121°C.

재구성

Stir to suspend 15.5g powder in 1L water. Autoclave for 15 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable