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Merck
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D5030

Sigma-Aldrich

Dulbecco′s Modified Eagle′s Medium

Without glucose, L-glutamine, phenol red, sodium pyruvate and sodium bicarbonate, powder, suitable for cell culture

동의어(들):

DME, DMEM

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About This Item

UNSPSC 코드:
12352207
NACRES:
NA.75

Quality Level

500

양식

powder

기술

cell culture | mammalian: suitable

성분

phenol red: no
NaHCO3: no
L-glutamine: no
HEPES: no
glucose: no
sodium pyruvate: no

배송 상태

ambient

저장 온도

2-8°C

일반 설명

Dulbecco′s Modified Eagle′s Medium (DME) is a modification of Basal Medium Eagle (BME) that has been formulated with a 4-fold higher concentration of amino acids and vitamins. It includes additional supplementary components. The DME formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. Modifying the medium with 4,500 mg/L glucose is optimal for culturing certain cell types.
The most basic formulation offered. This formulation is used by investigators who want to start with the essential components of DME, and have the flexibility to optimize the formula for their own application.

애플리케이션

Dulbecco′s Modified Eagle′s Medium has been used:
  • to culture the isolated flexor digitorum brevis (FDB) fibers to measure exogenous fatty acid (FA) utilization as part of oxygen consumption rate (OCR) measurements
  • to culture the isolated tibialis anterior (TA) muscle fibers for lactate measurements
  • to culture the human bone marrow mesenchymal stem cells for osteogenic differentiation to prepare a low-glucose medium to culture human hepatocellular carcinoma HepG2 cells

수량

Formulated to contain 8.3 grams of powder per liter of medium.

재구성

Supplement with 1.0 g/L glucose, 0.584 g/L L-glutamine, 3.7 g/L sodium bicarbonate.

기타 정보

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

관련 제품

제품 번호
설명
가격

08168

Timestrip Plus™ 8 °C

또한 이 제품과 함께 일반적으로 구입

제품 번호
설명
가격

보충제

제품 번호
설명
가격

픽토그램

Exclamation mark
GHS07

신호어

Warning

유해 및 위험 성명서

H319

Hazard Classifications

Eye Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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시험 성적서(COA)

Lot/Batch Number
0000415963
0000415963
0000355008
0000355010
0000326465

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문서 라이브러리 방문

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Cellular metabolic demands change throughout the cell cycle. Nevertheless, a characterization of how metabolic fluxes adapt to the changing demands throughout the cell cycle is lacking. Here, we developed a temporal-fluxomics approach to derive a comprehensive and quantitative view of
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