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Merck
모든 사진(1)

주요 문서

R1383

Sigma-Aldrich

RPMI-1640 Medium

With ʟ-glutamine, without glucose and sodium bicarbonate, powder, suitable for cell culture

동의어(들):

Roswell Park Memorial Institute 1640 medium

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About This Item

MDL number:
UNSPSC 코드:
12352207
NACRES:
NA.75

제품명

RPMI-1640 Medium, With L-glutamine, without glucose and sodium bicarbonate, powder, suitable for cell culture

양식

powder

기술

cell culture | mammalian: suitable

성분

L-glutamine: yes
phenol red: yes
sodium pyruvate: no
HEPES: no
NaHCO3: no

배송 상태

ambient

저장 온도

2-8°C

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일반 설명

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

애플리케이션

RPMI-1640 Medium has been used as a cell culture medium to culture human MCF-7 breast cancer cells and human castrate-resistant prostate cancer (CRPC) cell lines for various cell culture experiments. It has also been used to culture human islets to assess the oxidative stress response in primary human islets.

수량

Formulated to contain 8.4 grams of powder per liter of medium.

재구성

Supplement with 2.0 g/L sodium bicarbonate.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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