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Merck
모든 사진(1)

문서

R5886

SAFC

RPMI-1640 Medium

HEPES modification, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

의약품 제조

동의어(들):

Roswell Park Memorial Institute 1640 medium

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About This Item

MDL number:
UNSPSC 코드:
12352207
NACRES:
NA.75

product name

RPMI-1640 Medium, HEPES Modification, With 25 mM HEPES, without L-glutamine., liquid, sterile-filtered, suitable for cell culture

Quality Level

무균

sterile-filtered

형태

liquid

기술

cell culture | mammalian: suitable

불순물

endotoxin, tested

pH

>7.2

성분

L-glutamine: no
sodium pyruvate: no
HEPES: 25 mM
phenol red: yes
NaHCO3: yes

배송 상태

ambient

저장 온도

2-8°C

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일반 설명

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.
This RPMI-1640 medium is supplemented with HEPES. HEPES is a Good′s physiological buffer that helps provide a more stable medium pH than bicarbonate buffers.

애플리케이션

RPMI-1640 Medium has been used:
  • to culture human lymphoblastoid cell line cells
  • prostate cancer cells
  • monocytes from human blood

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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