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R7755

SAFC

RPMI-1640 Medium

AutoMod, without L-glutamine and sodium bicarbonate, powder, suitable for cell culture

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Synonym(s):
Roswell Park Memorial Institute 1640 medium
MDL number:

Quality Level

form

powder

technique(s)

cell culture | mammalian: suitable

components

sodium pyruvate: no
HEPES: no
NaHCO3: no
L-glutamine: no
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

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This Item
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SAFC

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R7755

RPMI-1640 Medium

RPMI-1640 Medium Modified, with sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

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R7509

RPMI-1640 Medium

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R5886

RPMI-1640 Medium

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R8755

RPMI-1640 Medium

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

sodium pyruvate: no
HEPES: no
NaHCO3: no
L-glutamine: no
phenol red: yes

components

phenol red: no
L-glutamine: no
HEPES: no
sodium pyruvate: no
NaHCO3: yes

components

L-glutamine: no
sodium pyruvate: no
HEPES: 25 mM
phenol red: yes
NaHCO3: yes

components

HEPES: no
phenol red: no
L-glutamine: yes
NaHCO3: no
sodium pyruvate: no

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

500

Quality Level

400

Quality Level

400

Quality Level

-

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Other Notes

Modified for autoclaving
Without L-glutamine and sodium bicarbonate.

Quantity

Formulated to contain 10.3 grams of powder per liter of medium.

Reconstitution

Supplement with 0.3 L-glutamine, 2.0 g/L sodium bicarbonate.

Legal Information

Auto-Mod is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Protocols

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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