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R7513

Sigma-Aldrich

RPMI-1640 Medium

Modified, with sodium bicarbonate, without methionine, cystine and L-glutamine, liquid, sterile-filtered, suitable for cell culture

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MDL number:
NACRES:
NA.75

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

HEPES: no
sodium pyruvate: no
phenol red: yes
L-glutamine: no
NaHCO3: yes

shipped in

ambient

storage temp.

2-8°C

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1 of 4

This Item
R1145R178051536C
vibrant-m

R7513

RPMI-1640 Medium

vibrant-m

R1145

RPMI-1640 Medium

vibrant-m

R1780

RPMI-1640 Medium

vibrant-m

51536C

RPMI-1640 Medium

form

liquid

form

liquid

form

liquid

form

liquid

components

HEPES: no
sodium pyruvate: no
phenol red: yes
L-glutamine: no
NaHCO3: yes

components

NaHCO3: no
phenol red: yes
sodium pyruvate: no
HEPES: no
L-glutamine: no

components

NaHCO3: 2 g/L
phenol red: no
glucose: 2 g/L (Dextro)
L-glutamine: 0.3 g/L

components

HEPES: 5958 mg/L
L-glutamine: 300 mg/L
NaHCO3: 2000 mg/L
phenol red: 5.310 mg/L

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

-

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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