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FG1215

Sigma-Aldrich

RPMI-1640 Medium

With L-alanyl-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

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Quality Level

sterility

sterile-filtered
sterile

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

sodium pyruvate: no
NaHCO3: yes
HEPES: no
phenol red: yes
stable glutamine: yes

shipped in

ambient

storage temp.

2-8°C

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This Item
R1145R4130R7509
vibrant-m

FG1215

RPMI-1640 Medium

vibrant-m

R1145

RPMI-1640 Medium

vibrant-m

R4130

RPMI-1640 Medium

vibrant-m

R7509

RPMI-1640 Medium

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

sterility

sterile-filtered

components

sodium pyruvate: no
NaHCO3: yes
HEPES: no
phenol red: yes
stable glutamine: yes

components

NaHCO3: no
phenol red: yes
sodium pyruvate: no
HEPES: no
L-glutamine: no

components

L-glutamine: yes
NaHCO3: no
phenol red: yes
HEPES: 25 mM
sodium pyruvate: no

components

phenol red: no
L-glutamine: no
HEPES: no
sodium pyruvate: no
NaHCO3: yes

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.

Application

RPMI-1640 Medium has been used to culture murine fibroblast cells. It has also been used to culture human ovarian and rat substantia nigra cells.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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