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R5158

Sigma-Aldrich

RPMI-1640 Medium

HEPES Modification, With 25 mM HEPES and L-glutamine, liquid, sterile-filtered, suitable for cell culture

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MDL number:

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, tested

pH

>7.2

components

phenol red: 0.0053 g/L
L-glutamine: 0.3 g/L
NaHCO3: 2 g/L
HEPES: 5.96 g/L
glucose: 2 g/L (Dextro)

shipped in

ambient

storage temp.

2-8°C

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This Item
R8005R4130R7388
Sigma-Aldrich

Sigma-Aldrich

R5158

RPMI-1640 Medium

SAFC

SAFC

R8005

RPMI-1640 Medium

SAFC

SAFC

R4130

RPMI-1640 Medium

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R7388

RPMI-1640 Medium

form

liquid

form

powder

form

powder

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, tested

impurities

endotoxin

impurities

-

impurities

endotoxin, tested

components

phenol red: 0.0053 g/L
L-glutamine: 0.3 g/L
NaHCO3: 2 g/L
HEPES: 5.96 g/L
glucose: 2 g/L (Dextro)

components

phenol red: 0.0053 g/L
L-glutamine: 0.3 g/L
HEPES: 3.5745 g/L
NaHCO3: no
glucose: 4.5 g/L (Dextro)

components

L-glutamine: yes
NaHCO3: no
phenol red: yes
HEPES: 25 mM
sodium pyruvate: no

components

HEPES: 20 mM
NaHCO3: no
sodium pyruvate: no
phenol red: yes
L-glutamine: yes

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.
This RPMI-1640 medium is supplemented with HEPES. HEPES is a Good′s physiological buffer that helps provide a more stable medium pH than bicarbonate buffers.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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1000309185

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