추천 제품
Quality Level
양식
liquid
사용
sufficient for 125 reactions
sufficient for 1500 reactions
sufficient for 500 reactions
특징
Long & Accurate PCR
dNTPs included: no
hotstart: no
농도
5 units/μL
기술
PCR: suitable
색상
colorless
입력
purified DNA
적합성
suitable for PCR
배송 상태
wet ice
저장 온도
−20°C
일반 설명
AccuTaq™ long and accurate (LA) DNA Polymerase is a combination of Taq DNA polymerase with a small amount of a polymerase with the 3′→5′ exonuclease activity necessary for proofreading. It enables the amplification of genomic targets larger than 20 kb. This blend increases the length of amplification products by using the proofreading polymerase to repair terminal misincorporations. This repair enables the polymerase to resume elongating the growing DNA strand. After the proofreading activity, the final products mostly have a blunt end.
애플리케이션
AccuTaq™ LA DNA Polymerase produces long DNA amplicons for:
- Mutation analysis
- Cloning genes
- DNA sequencing
- cDNA library generation
- Polymerase chain reaction (PCR)
- Direct PCR
특징 및 장점
- Increased fidelity, up to 6.5× that of Taq DNA polymerase
- Efficiently and accurately produce amplicons up to 22 kb on genomic templates and up to 40 kb on less complex templates such as lambda or bacterial DNA.
포장
The enzyme is provided with an optimized 10× reaction buffer for enhanced amplification of complex templates. A separate vial of DMSO is included. Addition of DMSO in the reaction at a final concentration of 1-4% may increase yield and improve reliability of the system with some complex PCR targets.
기타 정보
AccuTaq LA DNA polymerase is an optimized blend of Sigma′s high quality Taq DNA polymerase and a small amount of an additional polymerase that exhibits 3′→5′ exonuclease or proofreading activity. By blending the Taq with the right amount of this proofreading enzyme, misincorporation errors are corrected, producing PCR amplicons that are longer and more accurate.
Store AccuTaq LA DNA Polymerase and the 10x Buffer at –20 °C. Store DMSO at room temperature. Melting frozen DMSO at ~30 °C will not affect performance.
단위 정의
One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74°C.
법적 정보
Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
AccuTaq is a trademark of Sigma-Aldrich Co. LLC
관련 제품
제품 번호
설명
가격
신호어
Danger
유해 및 위험 성명서
예방조치 성명서
Hazard Classifications
Aquatic Chronic 3 - Resp. Sens. 1
Storage Class Code
10 - Combustible liquids
Flash Point (°F)
188.6 °F
Flash Point (°C)
87 °C
가장 최신 버전 중 하나를 선택하세요:
이미 열람한 고객
L S Waldron et al.
Applied and environmental microbiology, 75(1), 108-112 (2008-11-04)
Effective management of human cryptosporidiosis requires efficient methods for detection and identification of the species of Cryptosporidium isolates. Identification of isolates to the species level is not routine for diagnostic assessment of cryptosporidiosis, which leads to uncertainty about the epidemiology
D Ware et al.
Infection and immunity, 74(1), 352-361 (2005-12-22)
Polyamines such as putrescine, spermidine, and cadaverine are small, polycationic molecules that are required for optimal growth in all cells. The intracellular concentrations of these molecules are maintained by de novo synthesis and transport pathways. The human pathogen Streptococcus pneumoniae
Rafiya Sultana et al.
PloS one, 8(2), e55320-e55320 (2013-02-06)
The PE and PPE multigene families, first discovered during the sequencing of M. tuberculosis H37Rv genome are responsible for antigenic variation and have been shown to induce increased humoral and cell mediated immune response in the host. Using the bioinformatics
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Eugene J Gardner et al.
Nature communications, 10(1), 4630-4630 (2019-10-13)
Mobile genetic Elements (MEs) are segments of DNA which can copy themselves and other transcribed sequences through the process of retrotransposition (RT). In humans several disorders have been attributed to RT, but the role of RT in severe developmental disorders
문서
Long and accurate PCR applications address the needs for longer read lengths, greater fidelity and higher yields than that which can be achieved with Taq DNA polymerase.
Validate CRISPR gene editing experiments easily with Sigma-Aldrich® T7E1 mismatch detection kit, ensuring successful editing.
프로토콜
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.
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