F8924

Fibroblast Growth Factor-10 human

>97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• 동의어(들): FGF-10
• MDL number: MFCD01867039
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 생물학적 소스: human
• Quality Level: 100
• 재조합: expressed in E. coli
• 분석: >97% (SDS-PAGE)
• 형태: lyophilized powder
• 효능: 28 ng/mL
• 분자량: 19 kDa by SDS-PAGE
• 포장: pkg of 25 μg
• 저장 조건: avoid repeated freeze/thaw cycles
• 기술: cell culture | mammalian: suitable
• 불순물: endotoxin, tested
• UniProt 수납 번호: O15520
• 저장 온도: −20°C
• 유전자 정보: human ... FGF10(2255)

설명

일반 설명

FGF10 (fibroblast growth factor 10) is a member of the FGF protein family, which is a mitogen of epithelial origin and has a paracrine activity. It is produced by mesenchymal lineage cells, and acts as a ligand for FGFR, most notably FGFR2b, which is expressed by epithelial cells.

애플리케이션

Fibroblast Growth Factor-10 human is suitable for culturing of HUVEC cell line (human umbilical vein endothelial cells) to determine the anti-angiogenic properties of sFLT-1 (soluble fms-related tyrosine kinase-1).

생화학적/생리학적 작용

FGF10 (fibroblast growth factor 10) is involved in cell growth, morphogenesis and embryogenesis. It plays an essential role in initiating the formation of limb bud, lung morphogenesis and brain formation during embryogenesis. It acts as a mitogen, where it facilitates the regeneration of epithelia post-bladder urothelial injury. Thus, it might have future applications during bladder transplantation. Studies in Japanese population show that polymosphisms- rs339501, rs12517396, and rs10462070 are linked with severe myopia, and might act as susceptibility factors for the same. In lung cancer cells, FGFR4 reduces the stem cell/self-renewal property of cancer cells, and makes them more susceptible to chemotherapy.

물리적 형태

Lyophilized from a 0.2 μm filtered solution in 20 mM MOPS, 50 mM sodium sulfate, 1 mM DTT, 0.5 mM EDTA, pH 7.2, containing 50 μg bovine serum albumin per 1 μg cytokine.

분석 메모

The biological activity is measured by its ability to stimulate the proliferation of a monkey epithelial cell line 4MBr-5.

안전성 정보

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable