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Merck
모든 사진(1)

주요 문서

F9006

Sigma-Aldrich

Anti-Mouse IgG (whole molecule)–FITC antibody produced in goat

IgG fraction of antiserum, buffered aqueous solution

동의어(들):

Goat Anti-Mouse IgG (whole molecule)–Fluorescein isothiocyanate

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About This Item

MDL number:
UNSPSC 코드:
12352203
NACRES:
NA.46

생물학적 소스

goat

결합

FITC conjugate

항체 형태

IgG fraction of antiserum

항체 생산 유형

secondary antibodies

클론

polyclonal

형태

buffered aqueous solution

기술

direct immunofluorescence: 1:64

저장 온도

2-8°C

타겟 번역 후 변형

unmodified

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

IgG antibody consists of four subclasses : IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical κ or λ light (L) chains of 50 kDa and 25 kDa respectively. The chains are interlinked with a disulfide bond.
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
Anti-Mouse IgG (whole molecule)-FITC antibody is specific for mouse IgG. Goat anti-mouse IgG is conjugated to Fluorescein Isothiocyanate (FITC), Isomer I. Following conjugation, unbound FITC is removed by extensive dialysis.

면역원

Purified mouse IgG

애플리케이션

Anti-Mouse IgG (whole molecule)−FITC antibody produced in goat has been used in flow cytometry.
The antibody may be used for immunofluorescent staining of mouse spleen cells at a working dilution of 1:64. A working antibody dilution of 1:40 was used to stain the conidia of Botrytis cinerea for immunofluorescence. The antibody was also used for immunofluorescence of primary luteal cell cultures incubated with primary antibody against CD45 and rhizoids of Chara globularis incubated with anti-human spectrin antibody.

생화학적/생리학적 작용

IgG (immunoglobulin G) antibody has its function similar to IgM antibody in complement system activation. IgG participates in hypersensitivity type II and type III. It helps in opsonization, complement fixation and antibody-dependent cell-mediated cytotoxicity.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Veronica Lanza Cariccio et al.
mAbs, 8(4), 741-750 (2016-03-11)
There is a strong need for rapid and reliable epitope mapping methods that can keep pace with the isolation of increasingly larger numbers of mAbs. We describe here the identification of a conformational epitope using Phage-based Representation OF ImmunoLigand Epitope
Development of a monoclonal antibody-based immunodetection assay for Botrytis cinerea
Bossi R and Dewey FM
Plant Pathology, 41, 472-482 (1992)
M Braun
Plant physiology, 125(4), 1611-1619 (2001-04-12)
Spectrin-like epitopes were immunochemically detected and immunofluorescently localized in gravitropically tip-growing rhizoids and protonemata of characean algae. Antiserum against spectrin from chicken erythrocytes showed cross-reactivity with rhizoid proteins at molecular masses of about 170 and 195 kD. Confocal microscopy revealed
Y Figenschau et al.
Human reproduction (Oxford, England), 12(3), 523-531 (1997-03-01)
A simplified method for the preparation and long-term cultivation of granulosa-luteal cells in serum-free medium is described. The cells were harvested from women undergoing in-vitro fertilization, enriched by sedimentation and dissociated by enzymatic treatment. We demonstrated, by introducing a synthetic
N C Crespo et al.
Cell death and differentiation, 9(7), 702-709 (2002-06-12)
Recently, we have shown that the farnesyltransferase inhibitor FTI-2153 induces accumulation of two human lung cancer cell lines in mitosis by inhibiting bipolar spindle formation during prometaphase. Here we investigate whether this mitotic arrest depends on transformation, Ras and/or p53

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