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일반 설명
β-Glucuronidase is a tetramer belonging to the glycosidase family with a molecular weight of 29 kDa. It is composed of 74 kDa identical monomers. The pI and optimal pH of the enzyme is 4.8 and 6-7 respectively.
애플리케이션
β-Glucuronidase was used in the study of Cer-β-glucuronide synthesis and evaluation of β-glucuronidase(s) from Escherichia coli or intestinal segments to release the head group.
New Technical Article Comparing Performance of Different Enzymes
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about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Effective in the hydrolysis of steroid glucuronides.
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis
단위 정의
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay).
물리적 형태
β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.
Highly purified solution in 50% glycerol
신호어
Danger
유해 및 위험 성명서
예방조치 성명서
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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이미 열람한 고객
D H Kim et al.
Biological & pharmaceutical bulletin, 18(9), 1184-1188 (1995-09-01)
beta-Glucuronidase was purified 360-fold from Escherichia coli HGU-3, an human intestinal bacterium. The specific activity of the purified enzyme was 17.78 units/mg protein. The enzyme (M.W. 290000) is composed of four subunits (M.W. 72000) with a pI and optimal pH
E M Schmelz et al.
Cancer research, 59(22), 5768-5772 (1999-12-03)
Dietary sphingolipids inhibit chemically induced colon cancer in mice. The most likely mediators of this effect are the metabolites ceramide (Cer) and sphingosine, which induce growth arrest and apoptosis in transformed cells. Sphingolipids are digested in both the upper and
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To construct a lentiviral RNA interference system targeting heparanase (HPSE) based on miR30 and to test its silencing effect. Three heparanase-shRNA structures were designed based miR30. The targeting fragments were obtained by PCR, then inserted into the vector LV PP-GFP
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프로토콜
Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration.
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