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Merck
모든 사진(1)

주요 문서

G7646

Sigma-Aldrich

β-Glucuronidase from Escherichia coli

Type VII-A, lyophilized powder, 5,000,000-20,000,000 units/g protein, pH 6.8 (30 min assay)

동의어(들):

β-D-Glucuronide glucuronosohydrolase

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About This Item

CAS Number:
효소 위원회 번호:
EC Number:
MDL number:
UNSPSC 코드:
12352204
NACRES:
NA.54

유형

Type VII-A

Quality Level

양식

lyophilized powder

특이 활성도

5,000,000-20,000,000 units/g protein, pH 6.8 (30 min assay)

분자량

290 kDa

구성

Protein, ~25% biuret

저장 온도

−20°C

유사한 제품을 찾으십니까? 방문 제품 비교 안내

애플리케이션

New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Effective in the hydrolysis of steroid glucuronides.
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

품질

Contains buffer salts and stabilizer.

단위 정의

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay).

픽토그램

Health hazard

신호어

Danger

유해 및 위험 성명서

예방조치 성명서

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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시험 성적서(COA)

Lot/Batch Number

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문서 라이브러리 방문

Lawrence D Joh et al.
Biotechnology progress, 22(3), 723-730 (2006-06-03)
Extraction and storage of a recombinant protein produced by transient expression following agroinfiltration of lettuce were investigated. Lettuce leaves expressing beta-glucuronidase (GUS) were extracted by homogenization in several buffer combinations, and the yield and stability were assessed. The reducing agent
Optimized conditions for the emzymatic hydrolysis of a-hydroxytriazolam-glucoronide in human urine.
Tsujikawa, K., et al.
Health Laboratory Science, 40, 286-289 (2004)
H Mita et al.
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 34(8), 1262-1269 (2004-08-10)
Aspirin challenge of aspirin-intolerant asthma (AIA) patients causes a significant increase in leukotriene E4 (LTE4) concentration in urine. However, knowledge on leukotriene B4 (LTB4) generation in patients with AIA is insufficient. Recent research has demonstrated that exogenously administered LTB4 is
M Coulet et al.
Journal of veterinary pharmacology and therapeutics, 25(2), 89-97 (2002-05-10)
The pharmacokinetics of ibafloxacin, a new veterinary fluoroquinolone antimicrobial agent, was studied following intravenous (i.v.) and oral administration to healthy dogs. The mean absolute bioavailability of ibafloxacin after oral doses of 7.5, 15 and 30 mg/kg ranged from 69 to
Enzymes
Hans Ulrich Bergmeyer
Methods of Enzymatic Analysis, 460-461 (1974)

문서

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.

프로토콜

Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.

Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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