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Merck
모든 사진(1)

주요 문서

S1629

Sigma-Aldrich

Sulfatase from Aerobacter aerogenes

Type VI, buffered aqueous glycerol solution, 2-5 units/mg protein (biuret), 10-20 units/mL

동의어(들):

Aryl-sulfatase, Aryl-sulfate sulfohydrolase, Phenolsulfatase

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About This Item

CAS Number:
효소 위원회 번호:
MDL number:
UNSPSC 코드:
12352204
NACRES:
NA.54

유형

Type VI

Quality Level

양식

buffered aqueous glycerol solution

특이 활성도

2-5 units/mg protein (biuret)

분자량

~41 kDa

농도

10-20 units/mL

외래 활성

β-Glucuronidase ≤10 U/mL

배송 상태

wet ice

저장 온도

−20°C

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일반 설명

Sulfatases comprise Cys/Ser-X-Pro-X-Arg motif that is conserved in their active sites.

애플리케이션

Sulfatase from Aerobacter aerogenes has been used:
  • as a deconjugation enzyme for treating plasma samples for quercetin quantification using liquid chromatography with tandem mass spectrometry (LC/MS/MS) analyses
  • in fluorescence intensity-based enzymatic assay with an activity-based probe probe 1
  • to treat sulfatide liposomes to remove the 3-O-sulfogalactosyde head from sulfatides

생화학적/생리학적 작용

Sulfatases hydrolyze sulfate ester bonds to generate inorganic sulfates. Microbial sulfatases participate in sulfur scavenging and are essential enzymes for the utilization of sulfur. They may be associated with pathogenesis. Commercially available sulfatase from Aerobacter aerogenes is useful as a deconjugation enzyme for the removal of glucuronate and sulfate moieties from conjugates. Sulfatases find application in industry and agriculture.

단위 정의

One unit will hydrolyze 1.0 μmole of p-nitrophenyl sulfate per min at pH 7.1 at 37 °C.

물리적 형태

Solution in 50% glycerol containing 0.01 M Tris, pH 7.5.

기질

제품 번호
설명
가격

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서 라이브러리 방문

T Saidha et al.
Archives of biochemistry and biophysics, 272(1), 237-244 (1989-07-01)
Mitochondria that have been purified from cells of light-grown wild-type Euglena gracilis Klebs var. bacillaris Cori or dark-grown mutant W10BSmL and incubated with 35SO4(2-) and ATP accumulate a labeled compound in the surrounding medium. This compound is also labeled when
Melanie Glauser et al.
Clinica chimica acta; international journal of clinical chemistry, 430, 125-128 (2014-01-15)
Total (i.e. free+sulfated) metanephrines in plasma is a biomarker for the diagnosis of pheochromocytoma/paraganglioma. Sulfated metanephrines must be completely deconjugated by perchloric acid hydrolysis or sulfatase treatment prior to analytical measurement to enable quantification by current techniques. In this report
Toshiyuki Nakamura et al.
Bioscience, biotechnology, and biochemistry, 75(8), 1506-1510 (2011-08-09)
β-Glucuronidase and sulfatase are the major deconjugating enzymes used in the cleavage of the glucuronate and sulfate moieties, respectively, from certain conjugated food factors including polyphenols. In the present study, we found that compounds having the same molecular weights as
Toyokazu Miura et al.
Bioscience, biotechnology, and biochemistry, 70(6), 1509-1512 (2006-06-24)
Arylsulfatase activity was detected in a bacterial strain, Citrobacter braakii 69-b, isolated from soil by enrichment cultivation using porcine gastric mucin. The production of arylsulfatase was derepressed markedly in a synthetic medium by the addition of tyramine. The purified enzyme
Bioluminescent probes of sulfatase activity.
Jason S Rush et al.
Chembiochem : a European journal of chemical biology, 11(15), 2096-2099 (2010-09-28)

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