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Merck
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주요 문서

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G7663

Sigma-Aldrich

Gelatin blocking buffer

for Western blotting, powder blend

동의어(들):

blocking buffer

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About This Item

UNSPSC 코드:
41105300
NACRES:
NA.25

Grade

for molecular biology

Quality Level

100

양식

powder blend

저장 온도

room temp

일반 설명

Gelatin Blocking Buffer is a standard reagent in Western blotting procedures. It is used to block non-specific binding on the membrane, when using nylon or nitrocellulose (but not PVDF).

애플리케이션

Suitable for use as a non-specific blocking agent for Western blots.

재구성

Dissolve entire contents of the bottle in a final volume of 1L molecular biology grade water. The resulting solution will be faint yellow in color and does not require filtering or heating before use.

관련 제품

제품 번호
설명
가격

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
SLBR8508V
SLBP6251V
SLBN9526V
SLBD9944V
SLBD9944

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문서 라이브러리 방문

K A Brogden et al.
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Raul Bettencourt et al.
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A sensitive cell-based assay for the detection of residual infectious West Nile virus.
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Vaccine, 25, 39-39 (2007)
A Fortin et al.
Biochemistry and cell biology = Biochimie et biologie cellulaire, 72(5-6), 239-243 (1994-05-01)
The major advantages of the horseradish peroxidase chemiluminescence (HRP-CL) immunodetection method in Western blot analysis are its high sensitivity, nonradioactive detection, economy of the primary antibody, and speed of detecting the signal. However, we observed a strong and reproducible signal
Daisuke Tomida et al.
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프로토콜

Gelatin Blocking Buffer for Direct Detection Southern or Western Blotting

In order to specifically detect an antigen or target molecule immobilized on a solid support, unoccupied binding sites on the support must be blocked against binding by probe and detection molecules.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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