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H7033

Sigma-Aldrich

PerfectHyb™ Plus Hybridization Buffer

for Northern and Southern blotting, solution

동의어(들):

hyb buffer, hyb solution, hybridization solution, northern hybridization buffer, southern hybridization buffer

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About This Item

UNSPSC 코드:
12161700
NACRES:
NA.25

일반 설명

Sigma′s PerfectHyb Plus is a ready-to-use hybridization buffer for Northern and Southern blot protocols. Use of PerfectHyb Plus can decrease hybridization time to as little as two hours.

애플리케이션

Suitable for use in hybridization buffers used in Northern blot, Southern blot, colony/plaque blots, and dot blots.

특징 및 장점

  • Fast and easy-to-use
  • Up to 20x more sensitive than conventional buffers
  • Doesn′t require blocking steps
  • Compatible with neutral and positively-charged membranes
  • Compatible with chemiluminescent, colorimetric , and DIG detection assays
  • Compatible with radioactive and non-radioactive probes
  • Compatible with single or double-stranded DNA, RNA, and oligonucleotide probes

기타 정보

A small amount of precipitate that has formed in the solution can be dissolved at 30-35°C with occasional gentle inversion.

법적 정보

PerfectHyb is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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시험 성적서(COA)

Lot/Batch Number
0000287413
0000287414
SLCM5612
SLCJ9527
SLCD8886

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DIG Easy Hyb™ suitable for hybridization, solution

Roche

11603558001

DIG Easy Hyb™

Denhardt’s Solution for Northern and Southern blotting, lyophilized powder

Sigma-Aldrich

D9905

Denhardt′s Solution

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프로토콜

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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