추천 제품
생물학적 소스
Porcine heart
Quality Level
양식
lyophilized powder
특이 활성도
≥75 units/mg protein
구성
Protein, 75-95%
기술
cell culture | mammalian: suitable
색상
light yellow to yellow
외래 활성
L-glutamic dehydrogenase, lactic dehydrogenase and malic dehydrogenase ≤0.01%
glutamic-oxalacetic transaminase ≤0.1%
저장 온도
−20°C
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일반 설명
Glutamic-pyruvic transaminase (GPT) is localized majorly in the liver.
Research area: Cell Signaling
Research area: Cell Signaling
애플리케이션
Glutamic-Pyruvic Transaminase from porcine heart has been used:
- in the transamination assay with a racemic mixture of synthetic 3-methyl-2-oxoglutarate
- to remove glutamate from the cell culture medium used for culturing human embryonic kidney (HEK293) cells
- in the lactate-dehydrogenase (LD)-coupled alanine transaminase (ALT) reaction kinetic studies
생화학적/생리학적 작용
Glutamic-pyruvic transaminase (GPT) mediates the transfer of the amino group to α-ketoglutarate from l-alanine which results in pyruvate and glutamate.Thereby GPT plays a key role in gluconeogenesis and amino acid metabolism. The role of GPT in various kinds of tumors has been reported.
Glutamic-pyruvic transaminase (GPT) is located in most cells in the body, but is found in highest concentrations in the liver. It is used as a marker of liver disease due to the release of GPT into the blood following liver cell necrosis.
단위 정의
One unit will convert 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.6 at 37 °C in the presence of L-alanine.
물리적 형태
Lyophilized powder containing citrate and acetate
저장 및 안정성
Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized
persons
persons
분석 메모
Protein determined by biuret
신호어
Danger
유해 및 위험 성명서
예방조치 성명서
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
이미 열람한 고객
A Comprehensive Guide to Toxicology in Nonclinical Drug Development
Faqi A S
Biomarkers journal., 2017 null
A simple and sensitive detection of glutamic-pyruvic transaminase activity based on fluorescence quenching of bovine serum albumin
Chen M, et al.
Royal Society of Chemistry Advances, 5, 103557-103562 (2015)
Giordano Pula et al.
Journal of biochemical and biophysical methods, 64(3), 167-181 (2005-08-23)
The surface expression of G protein-coupled receptors is regulated by internalization. For many receptors, a constitutive level of internalization in the absence of agonist has been reported. The constitutive internalization of metabotropic glutamate receptor 1a (mGluR1a) has been described, but
Wei Guo et al.
Cancers, 12(7) (2020-07-15)
Metabolic reprogramming is an essential hallmark of cancer. Besides the "Warburg effect", cancer cells also actively reprogram amino acid metabolism to satisfy high nutritional demands in a nutrient-poor environment. In the glucose-alanine cycle, exogenous alanine taken up by hepatocytes is
Christoph Mahlert et al.
Journal of the American Chemical Society, 129(39), 12011-12018 (2007-09-06)
The acidic lipopeptides, including the calcium-dependent antibiotics (CDA), daptomycin, and A54145, are important macrocyclic peptide natural products produced by Streptomyces species. All three compounds contain a 3-methyl glutamate (3-MeGlu) as the penultimate C-terminal residue, which is important for bioactivity. Here
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