G9880

Glutamic-Pyruvic Transaminase from porcine heart

ammonium sulfate suspension, ≥75 units/mg protein

• 동의어(들): ALT1, L-Alanine: 2-Oxoglutarate Aminotransferase, Alanine Aminotransferase (catalytic concentration), GPT
• CAS Number: 9000-86-6
• 효소 위원회 번호: 2.6.1.2 (BRENDA, IUBMB)
• Beilstein: 3562140
• EC Number: 232-561-4
• MDL number: MFCD00131192
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: Porcine heart
• Quality Level: 200
• 양식: ammonium sulfate suspension
• 특이 활성도: ≥75 units/mg protein
• 분자량: 42 kDa
• 외래 활성: L-glutamic dehydrogenase, lactic dehydrogenase and malic dehydrogenase ≤0.01%; glutamic-oxalacetic transaminase ≤0.05%
• 배송 상태: wet ice
• 저장 온도: 2-8°C

설명

일반 설명

Research area: Cell Signaling
Glutamic-pyruvic transaminase (GPT) is located in most cells in the body but is found in highest concentrations in the liver. Two GPT isoforms, GPT1 and GPT2, are present in mammals.

애플리케이션

Glutamic-Pyruvic Transaminase from porcine heart has been used to determine L-lactate levels in aqueous polyethylene glycol (PEG) solutions.

생화학적/생리학적 작용

GPT is responsible for catalyzing the reversible transamination of α-ketoglutarate and alanine to glutamate and pyruvate. It also plays a critical role in the metabolism of amino acids and gluconeogenesis.

Glutamic-pyruvic transaminase (GPT) is located in most cells in the body, but is found in highest concentrations in the liver. It is used as a marker of liver disease due to the release of GPT into the blood following liver cell necrosis.

단위 정의

One unit will convert 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.6 at 37 °C in the presence of L-alanine.

물리적 형태

Suspension in 1.8 M (NH₄)₂SO₄ solution, pH 6.0

분석 메모

Protein determined by biuret.

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)