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Merck
모든 사진(4)

주요 문서

H0527

Sigma-Aldrich

HEPES potassium salt

≥99.5% (titration)

동의어(들):

Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-monopotassium salt, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid potassium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) potassium salt

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About This Item

실험식(Hill 표기법):
C8H17KN2O4S
CAS Number:
Molecular Weight:
276.39
MDL number:
UNSPSC 코드:
12161700
PubChem Substance ID:
NACRES:
NA.25

Quality Level

분석

≥99.5% (titration)

양식

powder

유용한 pH 범위

6.8-8.2

pKa 

7.5

solubility

water: 0.5 g/mL, clear, colorless

SMILES string

[K+].OCCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O4S.K/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

InChI key

KWURIFKZTJGMFD-UHFFFAOYSA-M

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일반 설명

HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid) is a widely used Good’s buffer in biochemical and biological research applications. As a zwitterionic buffer, it stabilizes the pH of the solution even when small amounts of acid or base are added.

애플리케이션

HEPES potassium salt has been used as a buffering agent in nanopore fabrication and electrolyte preparations. It has also been used as a component of cell lysis buffer.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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이미 열람한 고객

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Determining the location of an Arabidopsis chloroplast protein using in vitro import followed by fractionation and alkaline extraction
Chu CC and Li HM
Methods in Molecular Biology (2011)
Complexation of copper by zwitterionic aminosulfonic (good) buffers
Mash HE, et al.
Analytical Chemistry (2003)
Equilibrium studies, synthesis and characterisation of zwitterionic buffer (HEPES and HEPPS) dimethyltin (IV) complexes
Mohamed M
Main Group Chemistry, 63-84 (2007)
Use of a solid-state nanopore for profiling the transferrin receptor protein and distinguishing between transferrin receptor and its ligand protein
O'Donohue M, et al.
Electrophoresis (2023)
Jugal Saharia et al.
RSC advances, 11(39), 24398-24409 (2021-08-07)
Nanopore probing of molecular level transport of proteins is strongly influenced by electrolyte type, concentration, and solution pH. As a result, electrolyte chemistry and applied voltage are critical for protein transport and impact, for example, capture rate (C R), transport

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