H3537

HEPES solution

99.5%, liquid, BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered

• 동의어(들): N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
• CAS Number: 7365-45-9
• MDL number: MFCD00006158
• UNSPSC 코드: 12161700
• PubChem Substance ID: 329815199
• NACRES: NA.25

속성

• 제품명: HEPES solution, BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered
• Grade: BioPerformance Certified
• Quality Level: 200
• 무균: 0.2 μm filtered
• 양식: liquid
• 농도: 1 M
• 기술: cell culture | mammalian: suitable
• 불순물: Bioburden, tested; DNase, RNase, Protease, Nickase, free; endotoxin, tested
• pH: 5.0-6.0
• 유용한 pH 범위: 6.8-8.2
• 양이온 미량물: Fe: <5 ppm; heavy metals (as Pb): <5 ppm
• 적합성: suitable for molecular biology
• 응용 분야: diagnostic assay manufacturing
• SMILES string: OCCN1CCN(CCS(=O)(O)=O)CC1
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• InChI key: JKMHFZQWWAIEOD-UHFFFAOYSA-N

설명

일반 설명

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

애플리케이션

RNAse HEPES has been used:

• To supplement Dulbecco′smodified Eagle′smedium for maintenance of cell line and RPMI medium to wash rat islets
• To recover purified ribonucleotide
• As a component of HEPES/KOH buffer and adenylation buffer for small RNA isolation and sequencing
• As a component in buffers used for nuclear extract preparation and also to supplement RPMI-1640 medium for maintenance of islets

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable