51558

HEPES buffered saline

BioUltra, for molecular biology, 2× concentrate

• 동의어(들): HEPES Saline Buffer Solution
• MDL number: MFCD00006158
• UNSPSC 코드: 12161700
• PubChem Substance ID: 329757705
• NACRES: NA.25

속성

• Grade: for molecular biology
• Quality Level: 200
• 제품 라인: BioUltra
• 형태: liquid
• 품질: 2× concentrate
• 구성: dextrose, 2.0 g/L ; HEPES, 10 g/L ; KCl, 0.74 g/L ; NaCl, 16 g/L ; Na₂HPO₄.2H₂O, 0.27 g/L
• 농도: 2 ×
• 불순물: DNases, none detected; RNases, none detected; phosphatases, none detected; proteases, none detected
• refractive index: n20/D 1.339
• pH: 7.2 (25 °C)
• 양이온 미량물: Al: ≤1 mg/kg; Ba: ≤1 mg/kg; Bi: ≤1 mg/kg; Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; Li: ≤1 mg/kg; Mg: ≤1 mg/kg; Mn: ≤1 mg/kg; Mo: ≤1 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Sr: ≤1 mg/kg; Zn: ≤1 mg/kg
• λ: neat
• UV 흡수: λ: 260 nm A_max: 0.06; λ: 280 nm A_max: 0.04
• SMILES string: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• InChI key: JKMHFZQWWAIEOD-UHFFFAOYSA-N

설명

일반 설명

HEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.

애플리케이션

HEPES buffered saline has been used:

• for the preparation of plasmid DNA
• as a reagent to prepare transfection master mix for DNA construct vectors
• to develop hydrogel precursor for cell leakage assay

성분

concentration (in H₂O): 10 g/l HEPES; 16 g/l NaCl; 0.74 g/l KCl; 0.27 g/l Na₂HPO₄.2H₂O; 2.0 g/l dextrose

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves