RDD002

HEPES

≥99.5%, crystalline powder, anhydrous, Redi-Dri^™

• 동의어(들): 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
• 실험식(Hill 표기법): C₈H₁₈N₂O₄S
• CAS Number: 7365-45-9
• Molecular Weight: 238.30
• Beilstein: 883043
• EC Number: 230-907-9
• MDL number: MFCD00006158
• UNSPSC 코드: 12161700
• PubChem Substance ID: 329824215
• NACRES: NA.25

속성

• 제품명: HEPES, anhydrous, free-flowing, Redi-Dri^™, ≥99.5%
• Grade: anhydrous
• Quality Level: 100
• 제품 라인: Redi-Dri^™
• 분석: ≥99.5%
• 양식: crystalline powder
• 품질: free-flowing
• pH: 5.0-6.5 (25 °C, 238 g/L)
• 유용한 pH 범위: 6.8-8.2
• pKa(25 °C): 7.5
• solubility: water: 500 mg/mL, clear, colorless
• SMILES string: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• InChI key: JKMHFZQWWAIEOD-UHFFFAOYSA-N

설명

일반 설명

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

애플리케이션

HEPES has been used:

• In the novel vesicle-budding protocol as a component in stock solution and buffer solutions
• As a component in intracellular solution for the preparation of brain-slice recording
• As a component of wash buffer, elution buffer and desalting buffer during affinity protein purification
• To maintain the pH at 7 in thermal denaturation assay of purified protein
• In isocratic elution of protein using gel filtration
• Along with NaCl in sample preparation for EPR (Electron Paramagnetic Resonance) spectroscopy

법적 정보

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

안전성 정보

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable