H7523

HEPES

BioXtra, pH 5.0-6.5 (1 M in H₂O), ≥99.5% (titration)

• 동의어(들): 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
• 실험식(Hill 표기법): C₈H₁₈N₂O₄S
• CAS Number: 7365-45-9
• Molecular Weight: 238.30
• Beilstein: 883043
• EC Number: 230-907-9
• MDL number: MFCD00006158
• UNSPSC 코드: 12161700
• PubChem Substance ID: 24895796
• NACRES: NA.25

속성

• 제품 라인: BioXtra
• Quality Level: 400
• 분석: ≥99.5% (titration)
• 양식: powder
• 불순물: Insoluble matter, passes filter test
• 무기 잔류물(900 °C): ≤0.1% (as SO₄)
• 손실: ≤1.0% loss on drying, 110°C
• pH: 5.0-6.5 (1 M in H₂O)
• 유용한 pH 범위: 6.8-8.2
• pKa(25 °C): 7.5
• solubility: H₂O: 1 M, clear, colorless
• 음이온 미량물: chloride (Cl^-): ≤0.05%
• 양이온 미량물: Al: ≤0.0005%; As: ≤0.0001%; Ba: ≤0.0005%; Bi: ≤0.0005%; Ca: ≤0.001%; Cd: ≤0.0005%; Co: ≤0.0005%; Cr: ≤0.0005%; Cu: ≤0.0005%; Fe: ≤0.0005%; K: ≤0.005%; Li: ≤0.0005%; Mg: ≤0.0005%; Mn: ≤0.0005%; Mo: ≤0.0005%; Na: ≤0.005%; Ni: ≤0.0005%; Pb: ≤0.0005%; Sr: ≤0.0005%; Zn: ≤0.0005%
• 흡수: ≤0.035 at 280 in H₂O at 1 M; ≤0.045 at 260 in H₂O at 1 M
• 응용 분야: diagnostic assay manufacturing
• SMILES string: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• InChI key: JKMHFZQWWAIEOD-UHFFFAOYSA-N

설명

일반 설명

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

애플리케이션

HEPES has been used:

• As a component of homogenisation buffer used in protein sample preparation
• To neutralise PRP (platelet-rich plasma) solution as part of PRP preparation
• As a component in 2X PBS buffer, which is used in phosphate calcium transfection in cell line preparation
• As a supplement in RPMI 1640 medium used to culture human umbilical vein endothelial cells (HUVEC) and in M199 complete medium for ex-vivo treatment of testicular tissue.

기타 정보

Easily compare specifications for HEPES products with the HEPES specification table.

안전성 정보

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)