추천 제품
양식
suspension
Quality Level
기술
affinity chromatography: suitable
Matrix
Highly cross-linked 6% Beaded Agarose
용량
15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein)
저장 온도
2-8°C
일반 설명
HIS-Select High Flow Nickel Affinity Gel is an immobilized metal-ion affinity chromatography (IMAC) gel that is designed to specifically bind His-tag proteins (histidine containing proteins) in chromatographic systems with pressures up to 200 psi and a maximum linear flow rate of 3,000 cm/hr.
애플리케이션
Affinity gel is suitable for the purification of histidine tagged proteins (His-tag proteins) in native and denaturing conditions.
Nickel Affinity Gel for the purification of HIS TAG® proteins (histidine tagged proteins). HIS-Select® High Flow (HF) brings the superior selectivity of HIS-Select technology to a highly cross-linked agarose for higher flow rates and mechanical stability under pressure. HIS-Select HF is designed for production scale purification and FPLC™ applications. As with other HIS-Select products, the non-charged, hydrophilic linkage of the nickel-nitrilotriacetic acid (Ni-NTA) chelate group to the agarose ensures true one-step purification.
특징 및 장점
- High selectivity for higher purity
- Unique non-charged hydrophilic linkage reduced non-specific binding
- Binding Capacity for histidine-tagged protein (His-tag protein) is greater than 15 mg/mL
- Highly cross-linked agarose matrix allows for purification under higher pressures
- Binding under denaturing or non-denaturing conditions
- One-step purification
물리적 형태
1:1 suspension in a 30% ethanol solution
제조 메모
Ethanol must be removed prior to use. Wash affinity gel in deionized water to remove the ethanol, then equilibrate with equilibration buffer that is provided.
법적 정보
FPLC is a trademark of Cytiva
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
신호어
Danger
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 1A Inhalation - Flam. Liq. 3 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 - STOT SE 2
표적 기관
Eyes,Central nervous system, Respiratory Tract
WGK
WGK 3
Flash Point (°F)
88.0 °F
Flash Point (°C)
31.1 °C
개인 보호 장비
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
Obtaining of Agr2 Specific Antibodies and Determination of the Agr2 Protein Distribution Pattern during Early Embryonic Development and Tadpole Regeneration in Xenopus laevis
Ivanova, A.S. et al.
Russian journal of developmental biology, 49, 393-397 (2018)
Irving E Vega et al.
Frontiers in neuroscience, 12, 879-879 (2018-12-19)
Aggregation of the protein tau is a pathological hallmark of Alzheimer's disease (AD) and related disorders. However, the molecular mechanisms that lead to tau protein aggregation are still unclear. Previously, we showed that EFhd2 protein is associated with pathological aggregated
Jian Ming Khor et al.
Development (Cambridge, England), 146(16) (2019-07-25)
Alx1 is a conserved regulator of skeletogenesis in echinoderms and evolutionary changes in Alx1 sequence and expression have played a pivotal role in modifying programs of skeletogenesis within the phylum. Alx1 regulates a large suite of effector genes that control
Minoru Tanaka et al.
Nature chemical biology, 12(12), 1089-1096 (2016-10-25)
Cellular signaling is often propagated by multivalent interactions. Multivalency creates avidity, allowing stable biophysical recognition. Multivalency is an attractive strategy for achieving potent binding to protein targets, as the affinity of bivalent ligands is often greater than the sum of
Irving E Vega et al.
Frontiers in neuroscience, 13, 845-845 (2019-08-29)
The transition of tau proteins from its soluble physiological conformation to the pathological aggregate forms found in Alzheimer's disease and related dementias, is poorly understood. Therefore, understanding the process that modulates the formation of toxic tau oligomers and their conversion
관련 콘텐츠
친화성 또는 GST 풀다운, 직렬 친화성 정제(TAP), 공동면역침전법을 사용하여 in vitro 단백질-단백질 상호작용을 연구하기 위한 풀다운 분석, 시약, 프로토콜.
Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.