L0777

Lipase from Aspergillus oryzae

solution, ≥100,000 U/g, white, beige

• 동의어(들): AOL, Lipolase 100L
• CAS Number: 9001-62-1
• 효소 위원회 번호: 3.1.1.3.
• EC Number: 232-619-9
• MDL number: MFCD00131509
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: Aspergillus sp. (Aspergillus oryzae)
• Quality Level: 200
• 형태: solution
• 특이 활성도: ≥100,000 U/g
• 저장 조건: (Tightly closed. Dry)
• 기술: cell based assay: suitable
• 색상: beige; white
• UniProt 수납 번호: I8ABR3; I8ADK3
• 저장 온도: 2-8°C
• InChI: 1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
• InChI key: QWZUIMCIEOCSJF-CHHCPSLASA-N

설명

일반 설명

Research area: Cell Signaling

Lipase is derived from Aspergillus oryzae by ammonium sulfate precipitation and chromatography.Lipases or triacylglycerol acyl hydrolases are a group of hydrolase enzymes that are usually found in humans and animals with monogastric stomachs. Lipase enzymes are generally formed in the pancreas and stomach where they aid in fat and lipids digestion.(2)

애플리케이션

Lipase from Aspergillus oryzae has been used:

• as a control enzyme in activity assays
• as a component in lipolase stock solution for the hydrolysis experiments
• to incubate scaffolds for lipase-accelerated degradation experiments
• as the lipolytic enzyme standard to detect lipolytic enzymatic activity via chromogenic agar plates and zymography

생화학적/생리학적 작용

Lipase is widely used in several industries including food and pharmaceuticals. It mediates the hydrolysis of fats and oil. Lipase is utilized for peptide synthesis and in the detergent industry. It is active in the range of pH 2-5 and temperature between 30-50°C. Metal ions such as Fe²⁺, Fe^3+, and Cu²⁺ prevent the action of lipase. A less polar organic solvent is preferred for high stability.Lipases play a crucial role in digestion as well as the transportation and processing of dietary lipid substrates by catalyzing the hydrolysis of ester bonds in lipid substrates. The lipase from Aspergillus oryzae (AOL), a multipurpose biocatalyst can be used in the kinetic resolution of a biotin intermediate lactone,α-lipoic acid, and 1-phenylethanol. Additionally, it has the ability to stereoselectively catalyze the hydrolysis of ethyl 2-(4-hydroxyphenoxy) propanoate and its analogs, which are key intermediates in the production of aryloxyphenoxypropionate herbicides. AOL serves as a promising biocatalyst in the esterification of a series of short-chain acids and alcohols to produce flavor esters. It can be used to catalyze the esterification of lauric acid with aromatic alcohol-benzyl alcohol.

제조 메모

Produced by submerged fermentation of a genetically modified Aspergillus oryzae microorganism

법적 정보

A product of Novozyme Corp.

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)