L4025

Lyticase from Arthrobacter luteus

lyophilized powder, ≥200 units/mg solid

• 동의어(들): (1,3)-β-D-Glucan endohydrolase, 1,3-β-Glucan glucohydrolase, Bacterial lyticase, Lysing enzyme
• CAS Number: 37340-57-1
• MDL number: MFCD00131560
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: bacterial (Arthrobacter luteus)
• Quality Level: 200
• 양식: lyophilized powder
• 특이 활성도: ≥200 units/mg solid
• 기술: cell based assay: suitable
• 적합성: suitable for cell lysis
• 응용 분야: diagnostic assay manufacturing
• 저장 온도: −20°C

설명

애플리케이션

Lyticase from Arthrobacter luteus has been used for resuspending cells to extract high-molecular weight DNA for polymerase chain reaction (PCR) fingerprint analyses.

Lyticase from Arthrobacter luteus has been used:

• as a component in spheroplasting buffer to prepare yeast nuclear extracts
• for digestion in sample preparation for β-glucan enzymatic assay using yeast
• to lyse cells for RNA isolation

생화학적/생리학적 작용

Lyticase enzyme is frequently used in fungal research, particularly for species identification using polymerase chain reaction (PCR)-based techniques. It can break down β (1→3) and β (1→4) bonds between glucose units.

Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.

Lyticase is a lysing enzyme that is used to extract DNA from yeast cells by inducing partial spheroplast formation. Spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast, which are difficult to disrupt because the cell walls may form capsules or resistant spores. Lyticase contains β-(1→;3)-glucan laminaripentaohydrolase along with β-(1→3)-glucanase, protease, and mannanase activities. It is useful with yeast cells such as e.g. Candida, Debaryomyces, Saccharomyces, Saccharomycopsis, Saccharomycodes, Eremothecium, and Schwanniomyces species.

단위 정의

One unit will produce a ΔA₈₀₀ of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture.

기타 정보

For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)