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Key Documents

SRE0018

Sigma-Aldrich

Lyticase from Arthrobacter Luteus

Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, ≥2000 units/mg protein

Synonym(s):

Lyticase from Arthrobacter luteus

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About This Item

CAS Number:
37340-57-1
MDL number:
MFCD00131560
UNSPSC Code:
12352204
NACRES:
NA.32

biological source

bacterial (Arthrobacter luteus)

Quality Level

400

form

lyophilized powder

specific activity

≥2000 units/mg protein

composition

Protein, ≥20% biuret

application(s)

diagnostic assay manufacturing

shipped in

wet ice

storage temp.

−20°C

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Biochem/physiol Actions

Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.

Unit Definition

One unit will produce a ΔA800 of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Louis Dacquay et al.
G3 (Bethesda, Md.), 7(6), 1799-1809 (2017-04-30)
Actively proliferating cells constantly monitor and readjust their metabolic pathways to ensure the replenishment of phospholipids necessary for membrane biogenesis and intracellular trafficking. In Saccharomyces cerevisiae, multiple studies have suggested that the lysine acetyltransferase complex NuA4 plays a role in

Protocols

Enzymatic Assay of Lyticase

This procedure may be used for the determination of Lyticase activity using Baker’s yeast as the substrate.

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