L9504

D-Luciferin

luminescent, ≥98% (HPLC), powder

• 동의어(들): (S)-2-(6-Hydroxy-2-benzothiazolyl)-2-thiazoline-4-carboxylic acid, 4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid, Firefly Luciferin
• 실험식(Hill 표기법): C₁₁H₈N₂O₃S₂
• CAS Number: 2591-17-5
• Molecular Weight: 280.32
• Beilstein: 30484
• EC Number: 219-981-3
• MDL number: MFCD00042929
• UNSPSC 코드: 12352204
• PubChem Substance ID: 24896524
• NACRES: NA.32

속성

• product name: D-Luciferin, synthetic
• 생물학적 소스: synthetic
• Quality Level: 300
• 분석: ≥98% (HPLC)
• 형태: powder
• solubility: DMSO: 10 mg/mL, clear, colorless to faintly yellow
• 형광: λ_ex 328 nm; λ_em 532 nm in H₂O
• 저장 온도: −20°C
• SMILES string: OC(=O)[C@H]1CSC(=N1)c2nc3ccc(O)cc3s2
• InChI: 1S/C11H8N2O3S2/c14-5-1-2-6-8(3-5)18-10(12-6)9-13-7(4-17-9)11(15)16/h1-3,7,14H,4H2,(H,15,16)/t7-/m1/s1
• InChI key: BJGNCJDXODQBOB-SSDOTTSWSA-N

설명

일반 설명

D-Luciferin is a small molecule present abundantly in bioluminescent organisms. This molecule is sensitive to oxygen and light.

애플리케이션

D-Luciferin has been used in determining luciferase activity by luciferase assay in mice and Arabidopsis thaliana seeds. It has also been used in measuring bioluminescence in neonatal rat ventricular cardiomyocytes.

Substrate for firefly luciferase with a Km of approx 2 μM.

생화학적/생리학적 작용

D-Luciferin uptake within the cells is regulated by the adenosine triphosphate (ATP)-binding cassette (ABC) transporter inhibitors like ATP-binding cassette transporter G2 (ABCG2)/ breast cancer resistance protein (BCRP) inhibitor fumitremorgin C. This substrate is oxidized by firefly luciferase that results in emitting a photon, which has the capability of passing through living tissues.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)