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Merck
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주요 문서

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L9655

Sigma-Aldrich

Linoleic Acid-Oleic Acid-Albumin, 100x

liquid, sterile-filtered, BioReagent, suitable for cell culture

동의어(들):

Fatty Acid-Albumin-complexes

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About This Item

UNSPSC 코드:
12352211
NACRES:
NA.75

생물학적 소스

protein from bovine

Quality Level

300

무균

sterile-filtered

제품 라인

BioReagent

양식

liquid

구성

linoleic acid and oleic acid, 2 mol/mol albumin (each)

농도

100 mg/mL BSA in DPBS

기술

cell culture | mammalian: suitable

불순물

endotoxin, tested

저장 온도

2-8°C

일반 설명

Fatty Acid-Albumin-complexes have been employed as alternative sources of lipids in the development of serum-free media. Fatty acids bind to serum proteins in high proportions. Such proteins may release beneficial fatty acids and bind those that are inhibitory. Oleic acid bound to bovine serum albumin (BSA) is beneficial to the growth of a variety of cell types (e.g., baby hamster kidney (BHK) hybridoma). A mixture of poly-unsaturated and monosaturated fatty acids (i.e., linoleic acid and oleic acid) produces synergistic effect when used as a media supplement.

애플리케이션

Linoleic Acid-Oleic Acid-Albumin, 100x has been used:
  • to induce acute hepatocellular stress in primary hepatocytes
  • to induce oxidative stress in cytotrophoblasts
  • to induce lipid droplet accumulation in HepG2 cells

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관련 제품

제품 번호
설명
가격

08168

Timestrip Plus™ 8 °C

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves

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시험 성적서(COA)

Lot/Batch Number
0000315835
0000315835
0000294577
SLCP5387
SLCF7466

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문서 라이브러리 방문

Laura Moody et al.
Biochimica et biophysica acta. Gene regulatory mechanisms, 1862(2), 141-152 (2019-01-04)
Carnitine palmitoyltransferase 1 (Cpt1a) is a rate-limiting enzyme that mediates the transport of fatty acids into the mitochondria for subsequent beta-oxidation. The objective of this study was to uncover how diet mediates the transcriptional regulation of Cpt1a. Pregnant Sprague Dawley
Pierluigi Ramadori et al.
Free radical biology & medicine, 91, 114-126 (2015-12-25)
Generation of reactive oxygen species (ROS) in response to fatty acids accumulation has been classically proposed as a possible "second hit" triggering progression from simple steatosis to non-alcoholic steatohepatitis (NASH). In this study we challenged hepatocyte-specific Keap1 knockout mice (Keap1(Δhepa))
Anna Virginia Adriana Pirozzi et al.
Antioxidants (Basel, Switzerland), 10(1) (2021-01-06)
Several plant extracts are acquiring increasing value because of their antioxidant activity and hypolipidemic properties. Among them, great interest has been recently paid to açai fruit as a functional food. The aim of this study was to test the ability
Liang Tian et al.
International journal of molecular sciences, 20(5) (2019-03-13)
This study aimed to characterize the full-length cDNA of MARK4 in Sus scrofa, and evaluated its potential role in the regulation of lipid accumulation in pig placental trophoblasts and analyzed signaling pathways involved, thereby providing insights into mechanisms for placental
Yukimasa Takeda et al.
Scientific reports, 10(1), 3775-3775 (2020-03-01)
Brown adipocytes coordinate systemic energy metabolism associated with the pathogenesis of obesity and related metabolic diseases including type 2 diabetes. We have previously reported chemical compound-induced brown adipocytes (ciBAs) converted from human dermal fibroblasts without using transgenes. In this study
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문서

Linoleic acid in Cell Culture

Importance and uses of linoleic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

Oleic Acid in Cell Culture

How the unsaturated fatty acid, oleic acid and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies.

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