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Merck
모든 사진(1)

주요 문서

M4642

Sigma-Aldrich

Minimum Essential Medium Eagle

With Hanks′ salts and ʟ-glutamine, without sodium bicarbonate, powder, suitable for cell culture

동의어(들):

EMEM, MEM

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About This Item

UNSPSC 코드:
12352207
NACRES:
NA.75

product name

Minimum Essential Medium Eagle, With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

형태

powder

기술

cell culture | mammalian: suitable

성분

glucose: yes
Hanks’ salts (2% CO2): yes
L-glutamine: yes
Earle’s salts (5% CO2): no
NaHCO3: no
phenol red: yes
sodium pyruvate: no
stable glutamine: no
HEPES: no

배송 상태

ambient

저장 온도

2-8°C

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has high concentrations of amino acids, so the medium closely approximates the protein concentration of cultured mammalian cells. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has increased the applicability of this medium. This medium lacks calcium that permits the growth of cells in suspension. Modification of the original MEM formulation. Earle′s salts have been replaced with Hanks′ salts. Since the bicarbonate concentration is substantially lower, this medium has a substantially reduced buffering capacity.

애플리케이션

Minimum Essential Medium Eagle (MEM) has been used:

  • as a component of the Hank′s MEM (HMEM)-Pipes buffer to induce anaphylatoxin induced-ear swelling in mice
  • as a component of the slice culture medium (SCM) for the incubation of mouse hippocampal slice cultures
  • to culture Müller cells from guinea pigs

수량

Formulated to contain 10.6 grams of powder per liter of medium.

재구성

Supplement with 0.35 g/L sodium bicarbonate.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1


시험 성적서(COA)

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

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Journal of Neuroimmunology, 161, 49-60 (2005)
Mast cell anaphylatoxin receptor expression can enhance IgE-dependent skin inflammation in mice
Beatrix S, et al.
The Journal of Allergy and Clinical Immunology, 131(2), 541-548 (2013)
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Current biology : CB, 30(7), 1167-1176 (2020-02-23)
Synaptic plasticity, with its two most studied forms, long-term potentiation (LTP) and long-term depression (LTD), is the cellular mechanism underlying learning and memory. Although it has been known for two decades that bidirectional synaptic plasticity necessitates a corresponding bidirectional regulation
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문서

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

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