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M0769

SAFC

Minimum Essential Medium Eagle

AutoMod™, with Earle′s salts, without L-glutamine and sodium bicarbonate, powder, suitable for cell culture

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Synonym(s):
MEM
UNSPSC Code:
12171500
NACRES:
NA.75

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

NaHCO3: no
phenol red: yes
Earle’s salts (5% CO2): yes
L-glutamine: no
sodium pyruvate: no
HEPES: no

shipped in

ambient

storage temp.

2-8°C

Related Categories

General description

Modification of the original MEM formulation. Contains a succinate buffering system. See formulations table for specific information. Medium may be autoclaved.

Other Notes

Modified for autoclaving.

Quantity

Formulated to contain 9.4 grams of powder per liter of medium.

Reconstitution

Supplement with 0.292 g/L L-glutamine, 2.2 g/L sodium bicarbonate.

Legal Information

Auto-Mod is a trademark of Sigma-Aldrich Co. LLC

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Simona Giunta et al.
The Journal of cell biology, 190(2), 197-207 (2010-07-28)
The signaling cascade initiated in response to DNA double-strand breaks (DSBs) has been extensively investigated in interphase cells. Here, we show that mitotic cells treated with DSB-inducing agents activate a "primary" DNA damage response (DDR) comprised of early signaling events
Carlos Mendoza-Palomares et al.
Eukaryotic cell, 7(4), 684-697 (2008-02-19)
Cysteine proteases have been shown to be essential virulence factors and drug targets in trypanosomatids and an attractive antidisease vaccine candidate for Trypanosoma congolense. Here, we describe an important amplification of genes encoding cathepsin B-like proteases unique to T. congolense.
Hisashi Miura et al.
Nucleic acids research, 37(3), 778-792 (2008-12-17)
Mammalian tissue- and/or time-specific transcription is primarily regulated in a combinatorial fashion through interactions between a specific set of transcriptional regulatory factors (TRFs) and their cognate cis-regulatory elements located in the regulatory regions. In exploring the DNA regions and TRFs
Barbara Zonta et al.
Neuron, 69(5), 945-956 (2011-03-09)
The axon initial segment (AIS) is critical for the initiation and propagation of action potentials. Assembly of the AIS requires interactions between scaffolding molecules and voltage-gated sodium channels, but the molecular mechanisms that stabilize the AIS are poorly understood. The
Bo Ma et al.
Human vaccines & immunotherapeutics, 11(4), 998-1009 (2015-03-25)
Human diploid cell strains (HDCSs), possessing identical chromosome sets known to be free of all known adventitious agents, are of great use in developing human vaccines. However it is extremely difficult to obtain qualified HDCSs that can satisfy the requirements
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Articles

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

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Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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