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M2645

SAFC

Minimum Essential Medium Eagle

HEPES modification, with Earle′s salts and ʟ-glutamine, without sodium bicarbonate, powder, suitable for cell culture

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Synonym(s):
EMEM, MEM
UNSPSC Code:
12352207
NACRES:
NA.75

form

powder

technique(s)

cell culture | mammalian: suitable

components

HEPES: 25 mM
phenol red: yes
L-glutamine: yes
NaHCO3: no
sodium pyruvate: no
Earle’s salts (5% CO2): yes

shipped in

ambient

storage temp.

2-8°C

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Quantity

Formulated to contain 14.2 grams of powder per liter of medium.

Reconstitution

Supplement with 2.2 g/L sodium bicarbonate.

Other Notes

Leaving the bicarbonate out gives the investigator greater control over the buffer system.

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Seung Jin Han et al.
Scientific reports, 7(1), 14077-14077 (2017-10-28)
Control of protein turnover is critical for meiotic progression. Using RiboTag immunoprecipitation, RNA binding protein immunoprecipitation, and luciferase reporter assay, we investigated how rates of mRNA translation, protein synthesis and degradation contribute to the steady state level of Cyclin B1
Enrico Maria Daldello et al.
Development (Cambridge, England), 146(8) (2019-04-07)
Cyclins associate with cyclin-dependent serine/threonine kinase 1 (CDK1) to generate the M phase-promoting factor (MPF) activity essential for progression through mitosis and meiosis. Although cyclin B1 (CCNB1) is required for embryo development, previous studies concluded that CCNB2 is dispensable for
Cai-Rong Yang et al.
Nature communications, 11(1), 1399-1399 (2020-03-15)
Deleted in azoospermia-like (DAZL) is an RNA-binding protein critical for gamete development. In full-grown oocytes, the DAZL protein increases 4-fold during reentry into the meiotic cell cycle. Here, we have investigated the functional significance of this accumulation at a genome-wide
Pasqualino de Antonellis et al.
PloS one, 6(9), e24584-e24584 (2011-09-21)
Through negative regulation of gene expression, microRNAs (miRNAs) can function as oncosuppressors in cancers, and can themselves show altered expression in various tumor types. Here, we have investigated medulloblastoma tumors (MBs), which arise from an early impairment of developmental processes
Carlos Mendoza-Palomares et al.
Eukaryotic cell, 7(4), 684-697 (2008-02-19)
Cysteine proteases have been shown to be essential virulence factors and drug targets in trypanosomatids and an attractive antidisease vaccine candidate for Trypanosoma congolense. Here, we describe an important amplification of genes encoding cathepsin B-like proteases unique to T. congolense.

Articles

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

Protocols

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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