추천 제품
생물학적 소스
rabbit
Quality Level
결합
unconjugated
항체 형태
affinity isolated antibody
항체 생산 유형
primary antibodies
클론
polyclonal
형태
buffered aqueous glycerol solution
종 반응성
human (predicted), chicken
기술
western blot: 1:1,000 using chicken gizzard myosin II phosphorylated in vitro by PAK
배송 상태
dry ice
저장 온도
−20°C
타겟 번역 후 변형
phosphorylation (pSer19)
유전자 정보
human ... MYL1(4632)
일반 설명
Myosin light chains belongs to the calmodulin (CaM) gene family.
Rabbit polyclonal anti-phospho-Myosin Light Chain (pSer19) antibody detects chicken MLC. Results show that reactivity with the antibody requires phosphorylation of MLC by active kinase, and that only the peptide corresponding to MLC (pSer19) blocks the antibody signal, thereby demonstrating the specificity of the antibody. Human (100% homologous) has not been tested, but is expected to react.
면역원
synthetic phosphopeptide derived from the region of chicken MLC that contains serine 19 (serine 20 including the initiating methionine). Human (100% homologous) has not been tested, but is expected to cross-react.
애플리케이션
Anti-phospho-Myosin Light Chain (pSer19) antibody produced in rabbit has been used in the detection of phosphorylated myosin: in human lung fibroblasts and avian lens cells by immunoblotting by immunohistochemical detection in rabbit aorta samplesby immunofluorescence detection in human submandibular gland tissues
생화학적/생리학적 작용
Myosin light chain is a subunit of the conventional myosins, e.g., myosin II. In smooth muscle and nonmuscle cells, conventional myosins mediate a wide variety of contractile events including cytokinesis, cell motility, and smooth muscle contraction. MLC is phosphorylated by multiple serine-threonine kinases such as Rho-kinase and PAK, however, myosin light chain kinase (MLCK) acts as the primary kinase. Contractile activity of conventional myosins is regulated by phosphorylation of MLC on several residues. Phosphorylation of serine 19, the major phosphorylation site and the preferred site for MLCK, activates myosin motor activity under physiological conditions.
Myosin light chains functions to maintain integrity of myosin protein. Mutations in the myosin light chain is implicated in familial hypertrophic cardiomyopathy.
물리적 형태
Solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (± 0.1), 50% glycerol with 1.0 mg/mL BSA (IgG and protease free) and 0.05% sodium azide.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
The effects of actomyosin disruptors on the mechanical integrity of the avian crystalline lens
Won GJ et al.
Molecular Vision, 21(9), 98-98 (2015)
Shuichi Asano et al.
Physiological reports, 5(9) (2017-05-17)
In patients with pulmonary diseases such as idiopathic pulmonary fibrosis and severe acute respiratory distress syndrome, progressive pulmonary fibrosis is caused by dysregulated wound healing via activation of fibroblasts after lung inflammation or severe damage. Migration of fibroblasts toward the
Confocal microscopy with double immunofluorescence staining reveals the functional transient receptor potential vanilloid subtype 1 expressed in myoepithelial cells of human submandibular glands
Ding Q, et al.
Microscopy Research and Technique, 75(5), 555-560 (2012)
Matrix stiffness regulates migration of human lung fibroblasts
Asano S, et al.
Physiological Reports, 5(9), e13281-e13281 (2017)
Dorian Obino et al.
Cell reports, 25(11), 3110-3122 (2018-12-13)
Complete activation of B cells relies on their capacity to extract tethered antigens from immune synapses by either exerting mechanical forces or promoting their proteolytic degradation through lysosome secretion. Whether antigen extraction can also be tuned by local cues originating
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