M8042

Minimum Essential Medium Eagle

Alpha Modification, with nucleosides and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

• 동의어(들): EMEM, MEM
• UNSPSC 코드: 12352207
• NACRES: NA.75

속성

• 제품명: Minimum Essential Medium Eagle, Alpha Modification, with ribonucleosides, deoxyribonucleosides and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
• Quality Level: 400
• 무균: sterile-filtered
• 양식: liquid
• 기술: cell culture | mammalian: suitable
• 불순물: endotoxin, tested
• 성분: Hanks’ salts (2% CO₂): no; glucose: yes; sodium pyruvate: yes; phenol red: yes; HEPES: no; L-glutamine: no; stable glutamine: no; Earle’s salts (5% CO₂): yes; NaHCO₃: yes
• 배송 상태: ambient
• 저장 온도: 2-8°C

설명

일반 설명

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.This is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins. Often used in cytogenetic analysis when supplemented with fetal bovine serum (FBS).

애플리케이션

Minimum Essential Medium Eagle has been used for the isolation of mesenchymal stem cell, adipose-derived stem cell, and osteoblast.

Minimum Essential Medium Eagle is used for primary osteoblast cultures and for isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood.

생화학적/생리학적 작용

MEM usually autoclaved with 0.5% bactopeptone at 15 psi for 20 min, cooled and then neutralized with NaHCO₃, consistently supported good cell growth. A similar result was obtained when the MEM and bactopeptone were autoclaved separately. The cells grow, initially, as a monolayer and subsequently become a stationary suspension.

재구성

Supplement with 0.292 g/L L-glutamine

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable