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Merck
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문서

M4655

Sigma-Aldrich

Minimum Essential Medium Eagle

With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

동의어(들):

EMEM, MEM

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About This Item

UNSPSC 코드:
12352207
NACRES:
NA.75

무균

sterile-filtered

형태

liquid

기술

cell culture | mammalian: suitable

불순물

endotoxin, tested

성분

sodium pyruvate: no
phenol red: yes
Earle’s salts (5% CO2): yes
HEPES: no
NaHCO3: yes
L-glutamine: yes

배송 상태

ambient

저장 온도

2-8°C

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일반 설명

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle′s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.

MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.

애플리케이션

Generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera.

기타 정보

This product lacks L-Ala; L-Asn; L-Glu; Gly; L-Pro; L-Ser.

관련 제품

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가격

또한 이 제품과 함께 일반적으로 구입

제품 번호
설명
가격

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

시험 성적서(COA)

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문서

Calcium in Cell Culture

Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

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