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Merck
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주요 문서

MAK446

Sigma-Aldrich

Acid Phosphatase Assay Kit

Sufficient for 100 colorimetric tests

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About This Item

UNSPSC 코드:
12161503
NACRES:
NA.84

Quality Level

입력

serum
plasma
tissue sample(s)
cell lysate

검출 방법

colorimetric

관련 질환(들)

cancer, prostate tumor; gastrointestinal diseases

저장 온도

−20°C

일반 설명

Acid Phosphatase (ACP) is an enzyme which catalyzes the cleavage of phosphate groups from other molecules during digestion. Acid phosphatase can be found in lysosomes and become active after fusing with endosomes, acidifying the pH and, thus, creating an optimal environment for ACP. ACP can also be found in bone, spleen, liver, kidney and blood. Serum levels can be used as a biomarker for prostatic carcinoma, although prostate-specific antigen (PSA) is more widely used.

애플리케이션

The Acid Phosphatase Assay Kit may be used for:
  • Cancer Research
  • Gastrointestinal Research
  • Metabolism Research

특징 및 장점

Efficient and Precise: Requires only 20 μL of sample with a 30-minute assay reaction to detect acid phosphatase levels across a broad range from 0.05 to 60 U/L, providing both convenience and sensitivity.

Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

적합성

The kit is suitable for the determination of acid phosphatase in biological samples (e.g. plasma, serum, cell lysate, tissue samples.)

원리

The non-radioactive, colorimetric Acid Phosphatase Assay Kit is based on the cleavage of p-nitrophenol from the synthetic substrate. p-Nitrophenol becomes intensely colored after addition of the stop reagent. The increase in absorbance at A405 nm after addition of the stop reagent is directly proportional to the enzyme activity.

기타 정보

For additional information on our range of Biochemicals, please complete this form.

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

표적 기관

Nervous system,Kidney,Liver,Skin

Storage Class Code

8A - Combustible corrosive hazardous materials


시험 성적서(COA)

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