추천 제품
재조합
expressed in CHO cells
Quality Level
설명
contains a C-terminal 6-His tag
분석
≥90% (SDS-PAGE)
형태
solution
enzyme activity
≥15 units/mg protein
분자량
~61 kDa by SDS-PAGE (reducing)
포장
vial of 6-12 μg
불순물
≤1.0 EU/μg endotoxin
NCBI 수납 번호
배송 상태
dry ice
저장 온도
−70°C
유전자 정보
human ... NT5E(4907)
일반 설명
5′-Nucleotidase is an extracellular enzyme found in a wide variety of mammalian cells. It helps to accelerate the hydrolysis reaction of phosphate group from 5′ nucleotides, resulting in corresponding nucleosides.
생화학적/생리학적 작용
5′-Nucleotidase has various clinical significances. It is a key molecule in the regulation of cancer cells proliferation, migration and invasion in vitro tumor angiogenesis, and tumor immune escape in vivo. Due to this important role, the enzyme is a potential target for cancer research. It is also involved in salvage of extracellular nucleotides and plays a key role in the control of tissue homeostasis.
5′-nucleotidase, also known as CD73, is an extracellular enzyme that converts nucleoside-5′−monophosphates to nucleosides with a substrate preference of AMP. Native 5′-nucleotidase is a GPI-anchored protein whose exporession is upregulated by hypoxia. 5′-nucleotidase has many functions in vivo including the generation of extracellular adenosine.
단위 정의
One unit will hydrolyze 1.0 μmole of inorganic phosphorus from adenosine 5′-monophosphate per min at pH 9.0 at 37 °C.
물리적 형태
Supplied as a solution containing Tris, NaCl, CaCl2, and 20% glycerol.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Isolation of a plasma-membrane subfraction from rat liver containing an insulin-sensitive cyclic-AMP phosphodiesterase.
P D House et al.
European journal of biochemistry, 24(3), 429-437 (1972-01-21)
Zhao-wei Gao et al.
BioMed research international, 2014, 460654-460654 (2014-08-16)
Purinergic signaling has emerged as an important player in cancer progression and is regulated by a series of nucleotidases. Among the enzyme cascade, CD73, which catelyzes AMP breakdown to adenosine, has been found to be overexpressed in many types of
Anna Wilk et al.
Scientific reports, 10(1), 651-651 (2020-01-22)
Changes in nicotinamide adenine dinucleotide (NAD+) levels that compromise mitochondrial function trigger release of DNA damaging reactive oxygen species. NAD+ levels also affect DNA repair capacity as NAD+ is a substrate for PARP-enzymes (mono/poly-ADP-ribosylation) and sirtuins (deacetylation). The ecto-5'-nucleotidase CD73
Paul A Beavis et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(36), 14711-14716 (2013-08-22)
CD73 inhibits antitumor immunity through the activation of adenosine receptors expressed on multiple immune subsets. CD73 also enhances tumor metastasis, although the nature of the immune subsets and adenosine receptor subtypes involved in this process are largely unknown. In this
5'-Nucleotidase: molecular structure and functional aspects.
H Zimmermann
The Biochemical journal, 285 ( Pt 2), 345-365 (1992-07-15)
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