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Merck
모든 사진(4)

주요 문서

N2415

Sigma-Aldrich

Nucleoside Phosphorylase bacterial

recombinant, expressed in E. coli, ≥10 units/mg protein

동의어(들):

PNP, Purine nucleoside phosphorylase, Purine nucleoside:orthophosphate ribosyltransferase

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About This Item

CAS Number:
효소 위원회 번호:
EC Number:
MDL number:
UNSPSC 코드:
12352204
NACRES:
NA.54

재조합

expressed in E. coli

Quality Level

분석

≥80% protein basis (biuret)

양식

lyophilized powder

특이 활성도

≥10 units/mg protein

서열 기록

MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVRGMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLRDVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLFYSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAERQTTFNDMIKIALESVLLGDKE

UniProt 수납 번호

저장 온도

−20°C

SMILES string

[n]2(c3ncncc3nc2)[C@@H]1O[C@@H]([C@H]([C@H]1O)O)CO

InChI

1S/C10H12N4O4/c15-2-6-7(16)8(17)10(18-6)14-4-13-5-1-11-3-12-9(5)14/h1,3-4,6-8,10,15-17H,2H2/t6-,7-,8-,10-/m1/s1

InChI key

MRWXACSTFXYYMV-FDDDBJFASA-N

유전자 정보

Escherichia coli ... deoD(945654)

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애플리케이션

Bacterial nucleoside phosphorylase has been used in a study that identified and characterized two adenosine phosphorylase activities in Mycobacterium smegmatis. Bacterial nucleoside phosphorylase has also been used in a study to investigate the inhibitionof pyrimidine and purine nucleoside phosphorylases by a 3,5-dichlorobenzoyl-substituted 2-deoxy-D-ribose-1-phosphate derivative.
Nucleoside Phosphorylase bacterial has been used in reaction buffer and buffer-exchanged to remove residual phosphate.
Nucleoside phosphorylase is used in coupled enzyme systems to measure protein dephosphorylation.

생화학적/생리학적 작용

Nucleoside Phosphorylase/purine nucleoside phosphorylase (PNP), an enzyme participates in the purine salvage pathway. It also helps in the reversible cleavage of inosine to hypoxanthine and guanosine to guanine. PNP deficiency results in neurologic manifestations.
Catalyzes the following reaction:purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate

단위 정의

One unit will cause the phosphorolysis of 1.0 micromole of inosine to hypoxanthine andribose-1-phosphate per min at pH 7.4 at 25 deg C.

제조 메모

This is a salt-free and animal component-free enzyme preparation. This enzyme should be solubilized in a 10mM KPO4, pH 7.4 solution.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서 라이브러리 방문

Primary T-cell immunodeficiencies
Clinical Immunology (Orlando, Fla.), 54(7), 489-508 (2019)
Irina V Varizhuk et al.
Current protocols, 2(1), e347-e347 (2022-01-21)
A simple and efficient method for the preparation of α-D-ribose 1-phosphate and 2-deoxy-α-D-ribose 1-phosphate, key intermediates in nucleoside metabolism and important starting compounds for the enzymatic synthesis of various modified nucleosides, has been proposed. It consists in near-irreversible enzymatic phosphorolysis
Conformational stability and catalytic activity of PTEN variants linked to cancers and autism spectrum disorders
Johnston S B and Raines R T
Biochemistry, 54(7), 1576-1582 (2015)
Patricia Calero et al.
Nature communications, 11(1), 5045-5045 (2020-10-09)
Fluorine is a key element in the synthesis of molecules broadly used in medicine, agriculture and materials. Addition of fluorine to organic structures represents a unique strategy for tuning molecular properties, yet this atom is rarely found in Nature and
Disorders of purines and pyrimidines
Handbook of Clinical Neurology, 827-838 (2014)

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