추천 제품
제품명
o-Phenylenediamine dihydrochloride, tablet, 30 mg substrate per tablet
Quality Level
양식
tablet
mp
258 °C (dec.) (lit.)
solubility
water: 1 tablet/10 mL, clear, colorless
저장 온도
2-8°C
SMILES string
Cl[H].Cl[H].Nc1ccccc1N
InChI
1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H
InChI key
RIIWUGSYXOBDMC-UHFFFAOYSA-N
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일반 설명
o-Phenylenediamine dihydrochloride is a binucleophile and its condensation with α,β-unsaturated carbonyl compounds and aldehydes yields benzimidazoles.
o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.
애플리케이션
o-Phenylenediamine dihydrochloride has been used as a substrate for horse radish peroxidase in detecting:
- human immunoglobulin
- CTLA4Ig in serum samples
- albumin in culture medium
재구성
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.
신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
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시험 성적서(COA)
Lot/Batch Number
이미 열람한 고객
In vitro and in vivo properties differ among liquid intravenous immunoglobulin preparations
Dhainaut F, et al.
Vox Sanguinis, 104(2), 115-126 (2013)
Long-term graft acceptance in rat heart transplantation by CTLA4Ig gene transfection combined with FTY720 treatment
Ohba M, et al.
World Journal of Surgery, 25(4), 391-398 (2001)
The reaction of o-phenylenediamine with alpha, beta-unsaturated carbonyl compounds
Claramunt RM, et al.
ARKIVOC (Gainesville, FL, United States), 14, 35-45 (2006)
Acetic acid-promoted condensation of o-phenylenediamine with aldehydes into 2-aryl-1-(arylmethyl)-1H-benzimidazoles under microwave irradiation
Azarifar D, et al.
J. Serb. Chem. Soc., 75(9), 1181-1189 (2010)
Kazuo Ohnishi et al.
Japanese journal of infectious diseases, 65(1), 19-27 (2012-01-26)
The H5N1 subtype of the highly pathogenic (HP) avian influenza virus has been recognized for its ability to cause serious pandemics among humans. In the present study, new monoclonal antibodies (mAbs) against viral proteins were established for the
문서
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
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