R0525

RANK Ligand from mouse

≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• 동의어(들): Osteoclast differentiation factor (ODF), Osteoprotegerin ligand (OPGL), Receptor activator of NF-κB Ligand (RANKL), TNF-related activation−induced cytokines (TRANCE)
• MDL number: MFCD01867142
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 재조합: expressed in E. coli
• 분석: ≥98% (SDS-PAGE)
• 양식: lyophilized powder
• 효능: 10.0-25.0 ng/mL ED₅₀
• 포장: pkg of 10 μg
• 저장 조건: avoid repeated freeze/thaw cycles
• 기술: cell culture | mammalian: suitable
• 불순물: <0.1 ng/mg; <1 EU/μg
• 색상: white
• UniProt 수납 번호: O35235
• 저장 온도: −20°C
• 유전자 정보: mouse ... Tnfsf11(21943)

설명

일반 설명

Tumor necrosis factor (ligand) superfamily, member 11 (Tnfsf11) or receptor activator of nuclear factor-κB ligand (RANKL) is a type II transmembrane signaling receptor. It has a molecular weight of about 35kDa. The protein possesses an amino-terminal intracellular tail and a carboxy-terminal extracellular region, which has a connecting stalk and a receptor-binding domain.

애플리케이션

RANK ligand from mouse has been used to visualize differentiated osteoclasts and to stimulate osteoclast differentiation.

The functions of RANK Ligand include induction of activation of the c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function induction of osteoclastogenesis, and lymph node organogenesis.The cell surface signaling receptor of RANK Ligand is RANK, which undergoes receptor clustering during signal transduction.

생화학적/생리학적 작용

Tumor necrosis factor (ligand) superfamily, member 11 (Tnfsf11) or RANKL has an important role in osteoclast function and differentiation. It binds to RANK and osteoprotegrin (OPG). Bioactivities of RANKL may be attributed to its binding to these two receptor molecules. Mouse and human RANKL share 85% amino acid sequence identity.

물리적 형태

Lyophilized from a 0.2 μm filtered solution of 5 mM sodium phosphate, pH 7.6, and 75 mM NaCl.

분석 메모

The bioactivity is determined by its ability to induce osteoclast differentiation of RAW 264.7. The expected ED₅₀ for this effect is ≤10 ng/ml.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)