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Merck
모든 사진(1)

주요 문서

S1265

Sigma-Aldrich

Anti-Sheep IgG (whole molecule) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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About This Item

MDL number:
UNSPSC 코드:
12352203
NACRES:
NA.46

생물학적 소스

rabbit

Quality Level

결합

unconjugated

항체 형태

IgG fraction of antiserum

항체 생산 유형

secondary antibodies

클론

polyclonal

양식

buffered aqueous solution

기술

indirect ELISA: 1:50,000-1:100,000

배송 상태

dry ice

저장 온도

−20°C

타겟 번역 후 변형

unmodified

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일반 설명

Whole antiserum is fractionated and then further purified by ion exchange chromatography to provide the IgG fraction of antiserum. This fraction is essentially free of other rabbit serum proteins. Antiserum is determined to be immunospecific for sheep IgG by immunoelectrophoresis (IEP) versus sheep IgG and normal sheep serum. Identity and purity of the antibody is established by immunoelectrophoresis (IEP). Electrophoresis of the product followed by diffusion versus the anti-rabbit IgG and the anti-rabbit whole serum result in single arcs of precipitation in the γ region.

특이성

Binds all sheep Igs

면역원

purified sheep IgG

애플리케이션

Anti-Sheep IgG (whole molecule) antibody produced in rabbit has been used in:
  • Enzyme-linked immunosorbent assay (ELISA)
  • Immunoprecipitation
  • labelling electrodes in immunosensor systems

생화학적/생리학적 작용

Digestion of IgG by papain generates fragment antigen binding (Fab). Pepsin digestion of IgG results in fragment crystallisable (fc). IgG antibody has enormous therapeutic potential and the Fc is involved in the development of therapeutic antibody.

물리적 형태

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as preservative.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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