S9636

Superoxide Dismutase from human erythrocytes

essentially salt-free, lyophilized powder, ≥2,500 units/mg protein

• 동의어(들): SOD, Superoxide: superoxide oxidoreductase
• CAS Number: 9054-89-1
• 효소 위원회 번호: 1.15.1.1 (BRENDA, IUBMB)
• EC Number: 232-943-0
• MDL number: MFCD00132404
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: human erythrocytes
• Quality Level: 400
• 분석: >80% protein (biuret)
• 형태: essentially salt-free, lyophilized powder
• 특이 활성도: ≥2,500 units/mg protein
• 분자량: 32.0 kDa
• 구성: Protein, ≥80% biuret
• 제조업체/상표: Sigma-Aldrich
• 기술: activity assay: suitable
• 색상: white to off-white
• pH 범위: 7.6—10.5
• pH: 7.8
• 적합성: suitable for molecular biology
• UniProt 수납 번호: P00441; P04179; P08294
• 응용 분야: life science and biopharma
• 저장 온도: −20°C
• 유전자 정보: human ... SOD1(6647) , SOD2(6648) , SOD3(6649)

설명

일반 설명

Superoxide dismutases (SOD) are a group of low molecular weight metalloproteins present in all aerobic cells of plants, animals and micro-organisms. Three forms of SOD exist, based on the metal ions in the active site. These are Cu²⁺/Zn²⁺, Mn²⁺ and Fe²⁺ containing SOD. In vertebrate organisms, Cu/Zn-SOD is located in the cytoplasm as well as the mitochondrial intermembrane space, whereas Mn-SOD is located at the mitochondrial matrix space in prokaryotes. Fe-SOD is also found in prokaryotes and higher plants. Human erythrocyte SOD is a non-covalently bound homodimeric protein with two 16.3 kDa subunits containing 153 amino acids. Each dimer consists of two Cu²⁺ atoms and two Zn²⁺ atoms.

애플리케이션

Superoxide Dismutase from human erythrocytes has been used:

• to test its effect on human neutrophils in reactive oxygen species (ROS) measurement studies involving Pseudomonas aeruginosa infection
• as an antioxidant to test its effect on ROS generation induced by atmospheric-pressure plasma jet (APPJ) in red blood cell (RBC) homogenates using optical spectroscopy studies
• to test its attenuating effect on hemoglobin (Hb)-induced nuclear factor-kappa B (NF- κB) and hypoxia-inducible factor (HIF) activity in human dermal microvascular endothelial cells (HMECs-1)
• as a reference antioxidant protein to examine its expression in human intestinal Caco-2 cells following treatment with dietary flavonoids
• in combination with catalase to promote cell differentiation in vitro

생화학적/생리학적 작용

Mutations in the SOD1 gene are implicated in Amyotrophic lateral sclerosis (ALS).

Catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. Plays a critical role in the defense of cells against the toxic effects of oxygen radicals. Competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.

단위 정의

One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial ΔA₅₅₀ of 0.025 ± 0.005 per min.

분석 메모

For assay method, see McCord, J.M. and Fridovich, I., J. Biol. Chem., 244, 6049 (1969).

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)