C3556

Catalase from human erythrocytes

≥90% (SDS-PAGE), buffered aqueous solution, ≥30,000 units/mg protein

• 동의어(들): H₂O₂:H₂O₂ oxidoreductase
• CAS Number: 9001-05-2
• 효소 위원회 번호: 1.11.1.6 (BRENDA, IUBMB)
• EC Number: 232-577-1
• MDL number: MFCD00081483
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: human erythrocytes
• Quality Level: 200
• 분석: ≥90% (SDS-PAGE)
• 양식: buffered aqueous solution
• 특이 활성도: ≥30,000 units/mg protein
• 분자량: tetramer ~250 kDa
• 농도: ≤10 mg/mL
• 기술: cell based assay: suitable
• UniProt 수납 번호: P04040
• 배송 상태: wet ice
• 저장 온도: −20°C
• SMILES string: O(CC)C(=O)c1ccc(cc1)O
• InChI: 1S/C9H10O3/c1-2-12-9(11)7-3-5-8(10)6-4-7/h3-6,10H,2H2,1H3
• InChI key: NUVBSKCKDOMJSU-UHFFFAOYSA-N
• 유전자 정보: human ... CAT(847)

설명

일반 설명

Research area: Cell Signaling

Human catalase is a member of the monofunctional heme-containing catalases. It is an intracellular enzyme located at higher concentrations in the liver, erythrocytes, and kidney. Catalase is a homo-tetrameric protein and comprises amino acid residues, one heme group that is iron III protoporphyrin IX, and a nicotinamide adenine dinucleotide phosphate (NADPH) molecule. It is a ubiquitous enzyme found in most aerobic organisms. The catalase (CAT) gene is located on the human chromosome at 11p13.

애플리케이션

Catalase from human erythrocytes has been used:

• to prevent reoxidation of reduced cytochrome c by H2O2 while measuring the production of superoxide with cytochrome C.
• as a component of the imaging buffer for stochastic optical reconstruction microscopy (STORM) imaging of human skin fibroblasts
• as a component of the gloxy mix for single-molecule imaging

생화학적/생리학적 작용

Catalase activates the decomposition of hydrogen peroxide, a reactive oxygen species, into water and oxygen. It functions as a natural antioxidant, protecting cells against oxidative damage to proteins, lipids and nucleic acids. Catalase has also been used to study the role reactive oxygen species play in gene expression and apoptosis.

Deficiency of catalase and oxidative stress are associated with vitiligo, hypertension, Alzheimer′s disease, diabetes mellitus, and acatalasemia. Catalase shows a dichotomous role in cancer as its expression is at a higher level in chronic lymphocytic leukemia, glioma, melanoma, and gastric cancer and lower levels of expression are seen in acute myeloid leukemia, pancreatic, lung, prostate, and skin (non-melanoma) cancers.

단위 정의

One unit will decompose 1.0 μmole of H₂O₂ per min at pH 7.0 at 25 °C, while the H₂O₂ conc. falls from 10.3 to 9.2 mM, measured by the rate of decrease of A₂₄₀.

물리적 형태

Solution in 50 mM Tris, pH 8.0

저장 및 안정성

Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized
persons

분석 메모

Protein determined by biuret

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)