SAB4200760

Anti-Fibronectin Antibody

mouse monoclonal, FN-15

• 동의어(들): Anti-CIG, Anti-Cold-insoluble globulin, Anti-FN
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• product name: Anti-Fibronectin antibody, Mouse monoclonal, clone FN-15, purified from hybridoma cell culture
• 생물학적 소스: mouse
• Quality Level: 200
• 항체 형태: purified from hybridoma cell culture
• 항체 생산 유형: primary antibodies
• 클론: FN-15, monoclonal
• 형태: buffered aqueous solution
• 분자량: 220-260 kDa
• 종 반응성: human, mouse
• 농도: ~1 mg/mL
• 기술: immunoblotting: 0.3-0.6 μg/mL using human fetal fibroblast-like WI-38 cell line extract; immunofluorescence: 2.5-5 μg/mL using human foreskin fibroblast Hs68 cells; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: 0.15-0.3 μg/mL using 5 μg/mL Fibronectin from human plasma for coating
• 동형: IgG1
• UniProt 수납 번호: P02751
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... FN1(2335)

설명

일반 설명

Monoclonal Anti-Maltose Binding Protein (MBP) (mouse IgG1 isotype) is derived from the hybridoma MBP-17 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a purified recombinant MBP fusion protein. Fibronectin (FN), also known as Cold-insoluble globulin (CIG), is a multi-domain glycoprotein composed of two nearly identical disulfide-bound polypeptides. It is widely expressed by multiple cell types and is critically important in vertebrate development, as demonstrated by the early embryonic lethality of mice with targeted inactivation of the FN1 gene 6. In vertebrates, two types of Fibronectin are present: soluble plasma Fibronectin and insoluble cellular Fibronectin. The plasma form of Fibronectin is synthesized by hepatocytes, secreted to blood and, upon tissue injury, is incorporated into fibrin clots to exert effects on platelet function and to mediate hemostasis. Cellular Fibronectin is synthesized by many cell types, including fibroblasts, endothelial cells, chondrocytes, synovial cells and myocytes, it is assembled by cells as they migrate into the clot to reconstitute damaged tissue.

특이성

Anti-Fibronectin antibody, Mouse monoclonal specifically recognizes Fibronectin from plasma and in the pericellular extracellular matrix of cultured fibroblasts from human and mouse origin.

면역원

Fibronectin isolated from human plasma.

애플리케이션

The antibody may be used in various immunochemical techniques including Immunoblotting (∼220-260kDa)1, ELISA, Immunofluorescence, Immunohistochemistry and Immunoprecipitation.

생화학적/생리학적 작용

The plasma form of Fibronectin is synthesized by hepatocytes, secreted to blood and, upon tissue injury, is incorporated into fibrin clots to exert effects on platelet function and to mediate hemostasis. Cellular Fibronectin is synthesized by many cell types, including fibroblasts, endothelial cells, chondrocytes, synovial cells and myocytes, it is assembled by cells as they migrate into the clot to reconstitute damaged tissue.

물리적 형태

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

기타 정보

In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable